DNMT1和p27蛋白在原发性与继发性胶质母细胞瘤中的表达及相关性

Expression and significance of DNMT1 and p27 protein in primary and secondary glioblastoma multiforme

目的探讨原发性胶质母细胞瘤和继发性胶质母细胞瘤中DNA甲基转移酶1(DNMT1)蛋白及p27蛋白的表达及相关性。方法收集2000年1月1日至2012年12月31日经手术切除胶质母细胞瘤患者组织蜡块标本64例(原发胶质母细胞瘤32例,继发性胶质母细胞瘤32例)作为研究对象。检测64例胶质母细胞瘤标本和13例正常脑组织中DNMT1蛋白及p27蛋白的表达情况。结果在正常脑组织中,DNMT1蛋白不表达,而在原发性和继发性胶质母细胞瘤中的阳性表达率分别为59.4%和81.3%。在正常脑组织中,p27蛋白的阳性表达率为100.0%,高于其在原发性和继发性胶质母细胞瘤中的50.0%和25.0%(P<0.05)。DNMT1蛋白及p27蛋白在原发性和继发性胶质母细胞瘤中的表达差异存在统计学意义(P<0.05),但两者表达无相关性(r=0.41,P>0.05)。结论 DNMT1蛋白及p27蛋白在原发性和继发性胶质母细胞瘤中的表达存在差异,联合检测肿瘤标本中两者的表达情况有助于判断不同类型胶质母细胞瘤。

Objective To study the expression and correlation of DNA methyltransferase 1（ DNMT1） and p27 protein in primary and secondary glioblastoma multiforme. Methods From Jan 1 2000 to Jan 31 2012,32 cases of primary glioblastoma multiforme specimens,32 cases of secondary glioblastoma multiforme specimens and 13 cases of normal brain tissues were enrolled. The expression of DNMT1 and p27 protein was detected in those tissues. Results The positive expression rates of DNMT1 protein in primary and secondary glioblastoma multiforme tissues,as well as in normal tissues were 59. 4%,81. 3% and 0. The positive expression rates of p27 protein were 50. 0%,25. 0% and 100. 0% in primary and secondary glioblastoma multiforme tissues,and normal brain tissues（ P 0. 05）. The positive expression of DNMT1 and p27 protein in primary and secondary glioblastoma multiforme tissues had significant difference（ P〈0. 05）. There was no correlation between the expressions of both proteins（ r = 0. 41,P〉 0. 05）. Conclusion The expression of DNMT1 and p27 protein is different in primary and secondary glioblastoma multiforme tissues. The combined detection of DNMT1 and p27 protein may serve as an index to estimate different types of glioblastoma multiforme.