新疆哈萨克族高血压患者淋巴细胞白细胞介素6与其上游信号分子的表达

The expression of interleukin 6 and its upstream signals in Xinjiang Kazakh patients with essential hypertension

目的:观察哈萨克族高血压患者外周血淋巴细胞白细胞介素6(IL-6)和其上游信号T细胞核因子(NFAT)以及电压门控钾通道Kv1.3的表达情况,以明确淋巴细胞在高血压发生发展中的作用。方法:通过随机数字表法随机选取20例首次就诊且未经药物治疗的新疆哈萨克族高血压病患者作为高血压组,20例哈萨克族健康体检者作为对照组。每位入选者均采肘静脉血10ml,用密度梯度离心法分离出单个核细胞,再采用免疫磁珠法分离外周血T淋巴细胞。用逆转录聚合酶链反应法(RT-PCR)检测两组间mRNA的拷贝数,并用Western blot检测对应蛋白的表达量。结果:高血压组的IL-6[(1.04±0.52)︰(0.21±0.04)]、NFATC1[(6.08±0.66)︰(1.04±0.26)]和Kv1.3[(1.99±0.50)︰(0.96±0.40)]的mRNA表达量均高于对照组(均P<0.05);且高血压组的IL-6[(0.63±0.02)︰(0.19±0.03)]、NFATC1[(0.45±0.03)︰(0.27±0.05)]的蛋白表达量也明显高于对照组(均P<0.05)。结论:Kv1.3可能通过激活钙调磷酸酶/活化的NFAT信号通道引起炎性细胞释放,从而参与哈萨克族高血压疾病的发生。

Objective：To observe the expression of interleukin 6（IL-6）and its upstream signals named nuclear factor of activated T cells（NFAT）and voltage-gated potassium channels（Kv1.3）in Xinjiang Kazakh patients with essential hypertension and to deduce the function.Method：The 20 untreated Kazakh hypertensive patients and 20 Kazakh healthy subjects from Xinjiang were selected randomly in the study.T-lymphocytes were isolated from peripheral blood with magnetic cell sorting,the expression level of mRNA and protein＇s was detected by RT-PCR and Western blot analysis,respectively.Result：Compared with the control,the mRNA expressions of IL-6[（1.04±0.52）vs（0.21±0.04）],NFAT [（6.08±0.66）vs（1.04±0.26）]and Kv1.3 [（1.99±0.50）vs（0.96±0.40）]were significantly increased in Kazakh patients with essential hypertension（all P〈0.05）.The protein expression level of IL-6[（0.63±0.02）vs（0.19±0.03）]and NFATC1[（0.45±0.03）vs（0.27±0.05）]were also up-regulated markedly（both P〈0.05）.Conclusion：Kv1.3could produce inflammatory cytokines and then participate the pathological process of inflammation in essential hypertension through activating the CaN/NFAT signals in Xinjiang Kazakh patients.