多指标综合评分法优化痹痛消巴布膏提取工艺

Optimization for Extraction Technology of Bitongxiao Cataplasm by Multicriteria Comprehensive Assessment

目的优选痹痛消巴布膏的提取工艺。方法以挥发油提取率为指标,采用L9(34)正交法优化挥发油提取工艺;分别以丹酚酸B、阿魏酸,干浸膏得率为指标,采用L9(34)正交法优化药材水提取与醇提取工艺。结果最佳挥发油提取工艺为药材剪碎成颗粒加10倍量水提取3 h;最佳水提取工艺为提取2次,每次加水量15倍,每次提取时间为3 h;最佳醇提取工艺为乙醇回流法,提取3次,每次65%乙醇倍数为20倍,每次提取时间为2.5 h。结论采用多指标综合评分法优选的痹痛消巴布膏提取工艺合理、可行。

OBJECTIVE To optimize the extraction conditions of Bitongxiao Cataplasm. METHODS Orthogonal design L9（34） was used for the optimization of extraction conditions of essential oil guided by yield of essential oil. The orthogonal design L9（34） was carried out for the optimum water extraction conditions guided by yields of salvianolic acid B and ferulic acid. The orthogonal design L9（34） was carried out for the optimum ethanol extraction conditions guided by yield of dry extract. RESULTS The optimum extraction conditions of essential oil were determined with using 10 times of water and decocting 3 h. The optimum water extraction conditions were determined with using 15 times of water, decocting 2 times and 3 h each time. The optimum ethanol extraction conditions were determined with decocting 3 times, 20 times of 65% ethanol and 2.5 h each time. CONCLUSION The optimum extractions were reasonable and practical.