HPLC测定毛叶香茶菜中迷迭香酸和冬凌草甲素含量

Determination of Rosmarinic Acid and Oridonin in Rabdosia Japonica(Burm.f.) Hara by HPLC

目的建立高效液相色谱法测定毛叶香茶菜中迷迭香酸和冬凌草甲素含量的方法。方法采用Kromasil C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-0.1%磷酸为流动相,梯度洗脱,流速：1 m L·min^-1,检测波长：239 nm,柱温30℃。结果迷迭香酸和冬凌草甲素的线性范围分别为9.53-4 765 ng和13.66-6 830 ng,平均加样回收率分别为103.3%和95.8%,RSD%分别为1.4%和1.9%。结论该法简便、稳定、可靠、耐用性良好,可用于毛叶香茶菜的质量控制。

OBJECTIVE To develop a reversed phase high performance liquid chromatography method for simultaneous determination of rosmarinic acid and oridonin in Rabdosia japonica（Burm.f.） Hara. METHODS The separation of rosmarinic acid and oridonin were performed on a Kromasil C18 analytical column（250 mm×4.6 mm, 5 μm） with methanol（A）-0.1% phosphoric acid（B） as mobile phase in a gradient elution program at a flow rate of 1 m L·min^-1. The column temperature was maintained at 30 ℃. The detection wavelength was 239 nm. RESULTS Rosmarinic acid and oridonin had a good linearity ranging in 9.53-4 765 ng and 13.66-6 830 ng, respectively. The average recoveries of were 103.3% and 95.8%, RSDs were 1.4% and 1.9%, respectively. CONCLUSION The developed method is accurate with high simplify, stability, and reliability which is helpful to the quality control of Rabdosia japonica（Burm.f.） Hara.