姜黄素抑制哮喘大鼠气道平滑肌细胞TGFβ1表达的研究

Effects of curcumin on the airway smooth musle cell by inhibiting TGF β1 in asthmatic rats

目的观察姜黄素对哮喘大鼠气道平滑肌细胞(ASMC)增殖及TGFβ1表达的影响。方法将24只雄性SD大鼠分为对照组、哮喘组和姜黄素组。以卵清白蛋白(OVA)致敏激发建立哮喘大鼠气道重塑模型,图像分析技术测定支气管壁厚度(Wat)和平滑肌厚度(Wam),免疫组织化学法(IHC)测定肺组织TGFβ1蛋白表达。酶联免疫吸附法(ELISA)测定细胞培养液中TGFβ1含量,实时定量PCR(Real-time PCR)检测ASMC中TGFβ1m RNA表达,CCK-8分析ASMC增殖程度。结果姜黄素组与哮喘组相比,Wat及Wam厚度减少(P<0.01),仍高于对照组(P<0.01);IHC显示:姜黄素组肺组织中TGFβ1蛋白表达与哮喘组比较明显减少(P<0.01),仍高于对照组(P<0.01)。姜黄素组大鼠ASMC中TGFβ1和TGFβ1m RNA表达均降低,与对照组比较差异有统计学意义(P<0.01);姜黄素组ASMC增殖明显受到抑制(P<0.01)。结论姜黄素能抑制哮喘大鼠ASMC增殖,其机制与降低TGFβ1的表达有关。

Objective To study the role of curcumin on proliferation mechanism of airway smooth musle cell（ASMC）and TGFβ1 expression in asthmatic rats. Methods A total of 24 Sprague-Dawley（SD） rats were sensitized and challenged by OVA to establish asthmatic model were divided into three groups with 8 rats in each group. The total bronchial wall thickness（Wat） and the airway smooth musle thickness（Wam） were measured by image analysis system. The TGFβ1 expressions were determined by immunohistochemistry. Vitro experiments were conducted to determine the direct effect of curcumin on TGFβ1 expression by ASMC. Real-time PCR was employed to detect the expression of TGFβ1 m RNA levels. TGFβ1 concentrations were determined by sandwich enzyme-linked immunosorbent assay（ELISA）. Cell Counting Kit-8（CCK-8） was used to detect ASMC proliferation. Results Wat and Wam of curcumin group were significantly thiner than asthma group（P0.01）; but those were significantly thicker than control group（P0.01）. The TGFβ1 expressions in the curcumin group was significantly lower than those of the asthmatic group（P0.01）; but they were higher than the control group（P0.01）. Compared to control group, curcumin decreased the protein expressions and m RNA expressions of TGFβ1 of ASMC（P0.01）, inhibited ASMC proliferation（P0.01）. Conclusion Curcumin could inhibit ASMC proliferation in asthmatic rats, which is associated withthe decrease of TGFβ1.