颅脑损伤患者血清脑红蛋白含量与神经损伤程度的相关性研究

Relationship study between serum brain red protein content and the degree of nerve injury in patients with brain injury

目的 研究颅脑损伤患者血清脑红蛋白含量与神经损伤程度的相关性。方法 选择2012年4月~2015年10月在延安大学附属医院（以下简称“我院”）收治的38例颅脑外伤患者作为观察组,包括轻度损伤13例、中度损伤16例、重度损伤9例;选择同期在我院体检的40例健康志愿者作为对照组,采集两组患者的血清并测定脑红蛋白（NGB）、泛素羧基末端水解酶-L1（UCH-L1）、神经元特异性烯醇化酶（NSE）、S100β蛋白、胶质纤维酸性蛋白（GFAP）的含量,采用全面无反应性量表（FOUR）评价观察组患者的神经功能。结果 观察组患者的血清NGB[（325.27±55.11）比（57.33±8.15）pg/m L]、UCH-L1[（1.14±0.17）比（0.15±0.02）ng/m L]、NSE[（32.46±6.11）比（6.71±0.82）ng/m L]、S100β[（1.05±0.16）比（0.53±0.08）pg/m L]、GFAP[（22.52±4.18）比（10.69±1.73）pg/m L]含量显著高于对照组,差异均有统计学意义（P〈0.05）;颅脑损伤程度越严重,血清NGB、UCH-L1、NSE、S100β、GFAP的含量越高,睁眼反应、运动反应、脑干反射、呼吸节律评分越低（P〈0.05）;血清NGB含量与UCH-L1、NSE、S100β、GFAP含量均呈正相关（r=0.668、0.637、0.701、0.725,均P〈0.05）;血清NGB含量与睁眼反应、运动反应、脑干反射、呼吸节律评分均呈负相关（r=-0.628、-0.714、-0.683、-0.709,均P〈0.05）。结论 颅脑损伤患者血清NGB含量显著升高且与神经功能的损伤程度具有良好的相关性,是评估颅脑损伤严重程度理想的实验室指标。

Objective To study the relationship between serum brain red protein content and the degree of nerve injury in patients with brain injury. Methods 38 cases of traumatic brain injury patients in Affiliated Hospital of Yan＇an University（＂our hospital＂ for short） from April 2012 to October 2015 were selected as observation group, including 13 cases of mild injury, 16 cases of moderate injury and 9 cases of severe injury, 40 cases of healthy volunteers received physical examination in our hospital during the same period were selected as control group. Serum of two groups were collected and the contents of brain neuroglobin（NGB）, ubiquitin carboxy terminal terminal hydrolase L1（UCH-L1）, neuron specific enolase（NSE）, S100 beta protein, glial fibrillary acidic protein（GFAP） were determined. Neurological function of the observation group was determined by full outline of unresponsiveness. Results The contents of serum NGB [（325.27±55.11） vs（57.33±8.15） pg/m L）], UCH-L1 [（1.14±0.17） vs（0.15±0.02） ng/m L], NSE [（32.46±6.11） vs（6.71 ±0.82） ng/m L],S100 beta [（1.05±0.16） vs（0.53±0.08） pg/m L], GFAP [（22.52±4.18） vs（10.69±1.73） pg/m L] of the observation group were significantly higher than those in control group（P 0.05）, the more serious the degree of brain injury, the higher the contents of serum NGB, UCH-L1, NSE, S100-β, GFAP, the lower the scores of eyes reaction, motion response, brainstem reflex, respiratory rhythm（P 0.05）; content of serum NGB was positively correlated with UCH-L1, NSE, S100β,GFAP（r = 0.668, 0.637, 0.701, 0.725, all P 0.05）; content of serum NGB was negatively correlated with eyes reaction,motion response, brainstem reflex, respiratory rhythm（r =-0.628,-0.714,-0.683,-0.709, all P 0.05）. Conclusion Content of serum NGB of patients with traumatic brain injury is significantly increased, and has good correlation with the degree of nerve function injury, it is an ideal laboratory index to evaluate the severity of brain injury.