摘要
在15、18、21、24、27、30℃水温下,分别制备仿刺参福建连江群体和辽宁大连群体的体腔和内脏团提取液,测定超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、乳酸脱氢酶、碱性磷酸酶活力及丙二醛含量。试验结果显示,仿刺参连江群体内脏团提取液超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和乳酸脱氢酶活力最大时的水温比大连群体分别提高了3~9℃;高温(24、27、30℃)胁迫下,连江群体体腔和内脏团提取液超氧化物歧化酶、过氧化氢酶、乳酸脱氢酶活力均极显著高于大连群体(P〈0.01),其内脏团提取液谷胱甘肽过氧化物酶活力亦极显著高于大连群体(P〈0.01),其体腔液谷胱甘肽过氧化物酶活力在24℃和30℃时极显著高于大连群体(P〈0.01);仿刺参连江群体和大连群体体腔液碱性磷酸酶活性均在15℃时最大,内脏团提取液的碱性磷酸酶活性则均在18℃时最大;连江群体仿刺参体腔和内脏团提取液丙二醛含量在18~30℃下均显著低于大连群体(P〈0.05,P〈0.01)。研究表明,福建连江夏季高温驯化的仿刺参子二代群体,在高温胁迫试验中,其体腔和内脏团提取液中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、乳酸脱氢酶、碱性磷酸酶活力显著高于大连群体,丙二醛含量则显著低于大连群体,这些酶耐热性的提高,有利于仿刺参机体清除因高温胁迫产生多余的自由基,减少机体因高温胁迫造成的损害。
The activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX),lactate dehydrogenase(LDH),and alkaline phosphatase(AKP)and the content of malondialdehyde(MDA)in the coelomic fluid and extracting fluid of visceral mass were determined from Lianjiang and Dalian populations of sea cucumber,Apostichopus japonicus,exposed to different sea water temperature(15,18,21,24,27℃and 30℃).The results showed that the maximal activities of SOD,CAT,GSH-PX and LDH of extracting fluid of visceral mass were observde at 3—9℃ higher in Lianjiang population than in Daliang population;under high temperature(24,27,30℃)stress,the activities of SOD,CAT and LDH of coelomic fluid and extracting fluid of visceral mass population were very significantly higher in Lianjiang than in Daliang population(P〈0.01).The activity of GSHPX of extracting fluid of visceral mass was very significantly higher in Lianjiang population than in Daliang population(P〈0.01),and under 24℃and 30℃,the activities of GSH-PX of coelomic fluid population were also significantly higher in Lianjiang population than Daliang population(P0.01);the temperature of highest activity of AKP of coelomic fluid and extracting fluid of visceral mass from the two populations were all at 15℃and 18℃;the content of MDA of the coelomic fluid and extracting fluid of visceral mass was significantly lower in Lianjiang population than in Dalian population at 18—30℃(P〈0.05,P〈0.01).The findings of high temperature stress indicated that activities of SOD,CAT,GSH-PX and LDH of coelomic fluid and extracting fluid of visceral mass from the second generation of Lianjiang population of sea cucumber exposed to high temperature during summer in Fujian province,southeast China were significantly higher than Daliang population of sea cucumber;contents of MDA were lower significantly from Lianjiang population than Daliang population of sea cucumber.The increasing enzymes thermostability of the sea cucumber could be favorable to clean out excessive free radicals in the body under high temperature stress,and to protect the organism from high temperature stress damage.
出处
《水产科学》
CAS
CSCD
北大核心
2016年第4期327-333,共7页
Fisheries Science
基金
福建省科技重大专项项目(2011NZ0001-1)