温度、盐度和pH对仿刺参体腔细胞活性氧产生的影响

Effects of Water Temperature,Salinity and pH on Production of ROS by Coelomocytes of Sea Cucumber(Apostichopus japonicus)

无脊椎动物主要依靠天然免疫进行自身防御,而活性氧在保护宿主免受病原侵害方面发挥重要作用。本文研究了环境因子温度,盐度和pH对仿刺参体腔细胞吞噬过程中活性氧产生的影响。不同环境条件处理暂养7d的仿刺参,于不同时间点抽取体腔液,然后用鲁米诺化学发光法检测仿刺参体腔细胞体外吞噬酵母细胞时活性氧的产生。试验结果表明,与对照组相比(17℃),6℃和26℃水温中的仿刺参分别从第1d和第15d极大地增加了活性氧的产生。盐度25和盐度35两组仿刺参体腔细胞比对照组吞噬过程产生更多的活性氧,而盐度16和40两组第1d时活性氧的产生亦显著增强。较低pH同样极大增强了吞噬作用中活性氧的产生。研究结果表明,温度、盐度和pH均可影响仿刺参体腔细胞吞噬过程中活性氧的产生。该试验为进一步研究仿刺参在适应不同环境时的生理生化过程及无脊椎动物免疫机制奠定了基础。

Reactive oxygen species（ROS）play important roles in protection of host from pathogen in invertebrates which rely heavily on innate immune mechanisms to defence themselves.Here,the effects of environmental factors such as water temperature,salinity and pH on production of ROS by coelomocytes were studied in sea cucumber Apostichopus japonicus maintained under different environmental conditions.Coelomic fluid was extracted at different time points and then ROS production during in vitro phagocytosis of yeast cells by coelomocytes was measured 7dafter being acclimatization by the luminol chemiluminescence method.The results showed that the ROS production in sea cucumbers held in 6 ℃ and 26 ℃was dramatically increased from the first day and the fifth day compared with the control group（17 ℃）.There were in much more ROS in coelomocytes of the sea cucumbers cultured at salinity of 25 and 35generations than in the control group,while the same went for sea cucumbers kept at salinity of 16 and 40on the first day.Low pH also led to dramatically enhancement of the production of ROS during phagocytosis.In conclusion,water temperature,salinity and pH can influenced the production of ROS by the coelomocytes of sea cucumber during phagocytosis.Which lays a foundation for the further study of the physiological and biochemical processes of adaptation of sea cucumber to different environments as well as the innate immune mechanisms in invertebrates.