乌龟鲍曼不动杆菌的分离鉴定及药敏试验

Isolation,Identification and Drug Sensitivity of Acinetobacter baumannii from Tortoise Mauremys nigricans

自患病乌龟肝脏中分离出1株优势菌DL01221,对其进行形态学观察、生化试验、16SrRNA基因序列分析,同时采用Kriby-Bauer纸片扩散法进行药物敏感性试验。试验结果表明,该菌为革兰氏阴性球杆菌,将该菌株16SrRNA序列进行同源性和系统进化树分析,发现它与鲍曼不动杆菌处于同一群,同源性超过98%,结合菌株形态和生化指标,确定该菌株为鲍曼不动杆菌。致病性试验结果显示,其对乌龟具有较强的致病作用;耐药性结果显示其对头孢他啶、卡那霉素及氟罗沙星敏感,对其他多种抗生素耐药。

One predominant bacterial strain named DL01221 was isolated from the liver of diseased tortoise Mauremys nigricans,identified by morphologic observation,biochemical test,and sequencing analysis of16 SrRNA,and then the drug sensitivity was determind by Kirby-Bauer′s agar diffusion method.Results showed that the strain was gram negative bacterial.By analyzing the homology and phylogenetic relationship of 16 SrRNA sequences in the NCBI database,the homology was found to be more than 98% compared to Acinetobacter baumannii,which was in the same group with A.baumannii.Combining with the colonial morphology and biochemical results,the strain was identified as A.baumannii.Inoculating the strain to M.nigricans for lethality indicated that the strain had pathogenicity and the same bacterium could be isolated from these animals.Meanwhile the drug sensitivity test showed the bacterium was sensitive to ceftazidime,kanamycin and fleroxacin,and multi-resistant to most antibiotics.