珂字棉312组织培养体系的建立

Establishment of Tissue Culture System of Coker 312

以珂字棉312为试验材料,以无菌苗的下胚轴为外植体诱导愈伤,并将愈伤组织置于不同培养基下诱导分化,初步建立棉花组织培养体系。结果表明:一是对珂字棉312组织培养体系建立较好的各阶段培养基分别是愈伤组织诱导培养基为MS+0.1 mg/L 2,4-D+0.5 mg/L KT+30g/L葡萄糖+8 g/L琼脂,胚性愈伤组织的诱导培养基为MS+30 g/L葡萄糖+2.5 g/L phytagell,分化培养基为MS+0.1 mg/L IAA+30 g/L葡萄糖+2.5 g/L phytagell,生根培养基为MSB5(双倍KNO_3,无NH_4NO_3)+0.1 mg/L GA3+30 g/L葡萄糖+2.5 g/L phytagell;二是各培养基调节pH值为5.8,培养温度为28℃时能够获得植株再生体系。

Using the Coker 312 cotton cultivar as experimental material, We took its aseptic seedling＇ s hypocotyl as explant for callus induction, then took the callus into different culture medium for inducing differentiation and preliminarily built cotton tissue ! culture system. The results showed： first, the better culture mediums for the estabishment of the tissue culture system of Coker 312 in each stage were respectively that the callus - inducing medium was MS ＋ 0.1 mg/L 2, 4 - D ＋ 0.5 mg/L KT ＋ 30 g/L glucose ＋ 8 g/L agar, Induction medium of embryogenic callus was MS ＋ 30 g/L glucose ＋ 2.5 g/L phytagel, differential medium was MS ＋ 0.1 mg/L IAA ＋ 30 g/L glucose ＋ 2.5 g/L phytagel, rooting medium was MSB5 （ double KNO3, without NH4NO3 ） ＋ 0.1 mg/L GA3 ＋ 30 g/L glucose ＋ 2.5 g/L phytagel; second, the suitable pH value and culture temperature of culture medium for obtaining plant regeneration system were respectively 5.8 and 28℃.