摘要
目的:观察安坤种子丸对胚泡着床障碍小鼠雌、孕激素受体表达的影响,探讨其对胚泡着床障碍小鼠子宫内膜容受性的影响及作用机制。方法:筛选有规律动情周期的雌性小鼠,随机分为正常组、模型组和中药组,每组25只。用安坤种子丸先期对中药组小鼠进行灌胃,雌、雄合笼后观察雌鼠阴栓情况,之后各组选取有阴栓者20只并计为妊娠第1天(D1),雄鼠及其余雌鼠刎除。妊娠第4天对中药组和模型组注射米非司酮进行干预,制成胚泡着床障碍模型;于妊娠第5、6天处死小鼠,留取子宫标本。检测子宫雌激素受体(ER)、孕激素受体(PR)的表达;同时统计分析各组间妊娠率、平均着床胚泡数及胚泡着床率等指标的变化。结果:安坤种子丸可以提高胚泡着床障碍小鼠子宫内膜ER、PR的表达,妊娠率、平均着床胚泡数及胚泡着床率等指标虽低于正常组(P<0.05),但显著高于模型组(P<0.01)。结论:安坤种子丸可以提高雌鼠子宫内膜上ER、PR的水平,一定程度上改善着床障碍小鼠子宫内膜容受性,促进胚泡着床。
Objective： To observe the effect of Ankun Zhongzi wan on the expression of estrogen and endometrium progestrone receptor of rats with rmbryo implantation dysfunction,and to discuss its mechanism on endometrial receptivity of rats. Methods： Selected female rats with regular estrous cycle and divided into normal group, model group and Chinese medicine（CM）group,25 cases in each group. Gavages by Ankun Zhongzi wan was given to rats in CM group beforehand.Observed vaginal pessary of female rats after female and male rats were mated. On the pregnancy 1st day,intervention of injecting mifepristone was given to CM group and model group to make model of rmbryo implantation dysfunction,then rats were killed and uterine specimen was prepared on the pregnancy 5th and 6th day. Detected expressions of estrogen receptor（ER） and progestrone receptor（PR）,analyzed the change of pregnancy rates,number of blastocyst implantation,implantation rate and other indexes among all groups. Results：The expression of endometrium ER and PR of rats in Ankun Zhongzi wan group was improved, while indexes such as pregnancy rates, number of blastocyst implantation, implantation rate were lower than those in normal group（P〈0.05）, and were all higher than those in model group（P〈0.01）. Conclusion： Ankun Zhongzi wan can increase levels of endometrium ER and PR, improve endometrial receptivity of rats with implantation dysfunction on some degree,promote embryo implantation.
出处
《新中医》
CAS
2016年第7期286-289,共4页
New Chinese Medicine
基金
甘肃省自然科学基金课题(1308RJZA126)
