显微相衬技术分析去白细胞悬浮红细胞上清液不同存储期内细胞碎片及微粒的图像变化

Analysis on aleukocytic suspending red blood cell supernatant fragment and particle image changes at different stored periods by microscopic phase contrast technology

目的探索库存去白细胞悬浮红细胞,随着保存时间的延长,红细胞破损或溶血而产生的细胞碎片及微粒变化,为输血安全提供启示性实验基础。方法取不同保存天数(3、7、14、21d)的库存去白细胞悬浮红细胞进行上清液制备,通过显微静态图像分析技术对上清液中的微粒进行观察和形态分析。结果保存3d和7d的样品上清液中可见少量微粒,微粒大小和细胞相仿;从14d开始微粒的数量显著增加,微粒粒径更小,到21d微粒铺满了整个视场,呈现碎片化。结论保存期超过14d的库存去白细胞悬浮红细胞,其上清液的细胞碎片及微粒显著增多,较保存期不到14d的细胞均有显著的差异。这些外源细胞碎片或微粒可能会成为抗原并引起机体的免疫反应,导致输血不良反应发生,建议临床上给患者输注保存期在14d以内的去白细胞悬浮红细胞。

Objective To investigate the cellular fragments and particle image changes of inventory aleucocytic suspending RBC produced by the storage time extension,RBC damage or hemolysis in order to provide the revelatory experimental basis for the transfusion safety.Methods The supernatant was prepared from different stored days（3,7,14,21d）of stock aleukocytic suspending RBC.The particles in supernatant were observed and morphologically analyzed by using the microscopic static image analytic technology.Results There were a small amounts of visible particles in the sample supernatant preserved for 3,7dand the particles′sizes are similar to cells′;the number of particles began to significantly increase from 14 dand the diameter became smaller.The particles filled the entire field until 21 d,showing fragmentary status.Conclusion The cellular fragments and particles in the supernatants of stock aleukocytic suspending RBC with the storage period exceeding 14 dare significantly increased and have significant difference compared with those stored for less than 14 d.These exogenous fragments and particles may become antigens and induce the body immune response,lead to transfusion adverse reactions.It is recommended that the patients should be transfused with stock aleukocytic suspending RBC within a storage period of 14 d.