CRLR基因单核苷酸多态性与原发性前房角关闭人群的关联研究

Association of the single nucleotide polymorphisms in the calcitonin receptor-like receptor gene with primary angle closure in a Han Chinese population

目的:探讨降钙素受体样受体(calcitonin receptor-like receptor,CRLR)基因单核苷酸多态性(single nucleotide polymorpnism,SNP)与汉族原发性前房角关闭人群的关联性。方法:以流行病学人群为研究对象,采用病例对照设计。收集江苏省阜宁县流行病学调查中筛查出的原发性前房角关闭(primary angle closure,PAC)患者232例,正常对照306例。血样经DNA提取后采用TaqMan-MGB荧光探针法检测CRLR基因的rs1157699(C/T)位点SNP基因型,比较两组等位基因及基因型频率的分布。结果:病例组的基因型分布(CC 67.4%,CT 30.0%,TT2.6%),对照组的基因型分布(CC 71.3%,CT 27.0%,TT1.7%),两组之间差异无统计学意义(P>0.05)。结论:中国汉族人群的CRLR rs1157699位点SNP与原发性前房角关闭无相关性。

AIM： To study the association of the single nucleotide polym orphism（ SNP） rs1157699 in the calcitonin receptor-like receptor（ CRLR） gene with prim ary angle closure（ PAC） in a Han Chinese population.METHODS： All samples,involved 232 PAC cases and306 controls,were obtained from an epidem iologic survey conducted in Funing, Jiangsu Province, China.Genotyping were carried out by Taq Man-MGB probe using the real tim e quantitative polym erase chain reaction system to study the relationship between SNP of rs1157699 in CRLR gene and PAC.RESULTS： The prevalence of CRLRrs1157699 genotype was 67. 4%,30. 0%,2. 6% for CC,CT,TT in cases,and71. 3%,27. 0%,1. 7% in controls respectively. There was no difference between the two groups in the distribution of genotype and allele frequencies of rs1157699（ P〉0. 05）.CONCLUSION： Our results do not support a significant role for rs1157699 in CRLR with PAC.