Keap1在食管鳞状细胞癌中的表达及意义

Expression and clinical significance of Keap1 in esophageal squamous cell carcinoma

目的探讨食管鳞状细胞癌中Keap1(Kelch-like epichlorohydrin-associated protein 1,Keap1)的表达及临床意义。方法应用免疫组织化学SP法检测Keap1蛋白在62例手术切除食管癌组织及34例癌旁正常组织的表达情况,并分析其与临床病理特征及预后之间的关系。结果 Keap1在食管癌旁正常组织中达率明显高于食管癌组织(P<0.05),Keap1蛋白的表达与患者年龄、性别、肿瘤分化程度无关(P>0.05),与局部淋巴结是否转移、TNM分期有关(P<0.05),有局部淋巴结转移组Keap1蛋白的表达水平明显高于无局部淋巴结转移组,TNM分期越晚,Keap1蛋白表达水平越高;生存分析显示Keap1的表达与OS无关(P>0.05)。结论食管癌组织中Keap1蛋白表达下调,与食管癌局部淋巴结转移及TNM分期相关,Keap1可能参与了食管癌的发生发展和转移,有望成为食管鳞癌新的研究靶点。

Objective To investigate the expression and clinical significance of Keapl （Kelch - like epichlorohydrin - as- sociated protein 1, Keapl ） in esophageal squamous cell carcinoma （ESCC）. Methods The expression of Keapl in 62 ca- ses of ESCC tissues and 34 cases of adjacent normal tissues was detected and the correlation between Keapl expression and clinical pathological features and prognosis was analyzed by using SP immunohistochemical method. Results The positive expression rate of Keapl in adjacent normal tissues was significantly higher than that in ESCC （P 〈 0. 05）. The expression of Keapl was not related to the age, sex, tumor grades（P 〉0. 05） ,and it was correlated with the lymph node metastasis and TNM stages（P 〈0. 05）, the expression rate of Keapl was higher in the lymph node metastasis than that in the non - lymph node metastasis, the later the TNM stage, the higher the expression rate. Survival analysis showed that the expres- sion of Keapl was not related with the overall survival （P 〉 0. 05 ）. Conclusion The Keapl expression is down - regulated in ESCC, and the decreased expression is correlated with the lymph node metastasis and TNM stages. Keapl may partici- pate in the occurrence and development of ESCC, and may be utilized as a new research target of ESCC.