甲基强的松龙冲击治疗大鼠早期脊髓不全损伤的磁共振波谱分析

The magnetic resonance spectroscopy(MRS) analysis of early incomplete spinal cord injury treated by Methylprednisolone pulse in rats

目的用磁共振波谱(MRS)技术检测大鼠脊髓损伤,分析不同时段甲基强的松龙冲击治疗大鼠早期脊髓不全损伤神经化学代谢的改变。方法采用Benchmark TM颅脑撞击器建立大鼠脊髓损伤模型,甲基强的松龙组(实验组)在伤后1、3、6、8 h经尾静脉注射甲基强的松龙(30 mg/kg,随后以每小时5.4 mg/kg维持治疗24 h);对照组不做甲基强的松龙冲击治疗干预;2组分别在伤后1、3、7、14 d行脊髓MRS检查,并对同阶段损伤脊髓灰质右侧前角分析峰下面积比值与对照组比较,即NAA/H20、Cr/H20、Cho/H20,并行统计学分析。结果甲基强的松龙组冲击疗法MRS与对照组比较,差异无统计学意义(P>0.05)。结论 MRS能评价脊髓损伤,脊髓不全损伤早期大剂量甲基强的松龙冲击治疗,而不能减轻神经元继发性损害。

Objective Using the magnetic resonance spectroscopy （MRS） technique to detect the spinal cord injury of rats in order to analysis the neurochemical metabolism changes of early incomplete spinal cord injury in rats treated by Methylprednisolone pulse in different phase. Methods Using the Benchmark TM head impactor to establish the model of rat spinal cord injury, Methylprednisolone were injected at 1,3,6,8 hours respectively after injury （30 mg/kg, then 5.4 mg/kg maintenance treatment for 24 hours） ;The control group was not intervened by methylprednisolone. Spinal cord MRS examination was detected after, 1,3,7,14 days of injury, and the peak area ratio of the spinal cord grey matter right anterior horn at the same stage were analyzed of： NAA/H20, Cr/H2O, Cho/H2O. The datas were statistically significantby chi - square analysis （ P 〈 0.05 ）. Results Methylprednisolone pulse therapy of MRS had no statistical significance （P 〉 0.05）. Conclusion MRS can evaluate the spinal cord injuryA large dose of methylprednisolone can not reduce the secondary neuron injury of incomplete spinal cord injury in early stage.