IL-10基因启动子-592A/C多态性与胃癌发病风险的病例对照研究

A Case-Control Study of Interleukin-10 Gene-592A/C polymorphism and the Risk of Gastric Cancer

目的探讨白细胞介素-10基因启动子-592A/C多态性与宁夏地区汉族人群胃癌发病风险的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对147例胃癌患者(病例组)和150例健康对照组IL-10基因启动子-592A/C多态性位点进行检测。结果 IL-10基因SNP-592A/C的AA、AC、CC基因型在病例组中的分布频率分别为45.58%、42.86%和11.56%,在对照组中的分布频率分别为47.33%、44.67%和8.00%,2组的基因型分布频率差异无统计学意义(P>0.05)。IL-10基因SNP-592位点A、C等位基因在病例组中的分布频率分别为67.01%和32.99%,在对照组中的分布频率分别为69.67%和30.33%,2组的等位基因分布频率差异亦无统计学意义(P>0.05)。结论 IL-10基因启动子-592A/C位点可能与宁夏地区汉族人群胃癌的发病风险无相关性。

Objective To investigate the relationship between polymorphism of interleukin - 10 （IL - 10 ） gene promoter - 592A/C and the risk of gastric cancer. Method The gene IL - 10 - 592A/C polymorphisms was examined by the polymerase chain re- action restriction fragment length polymorphism （PCR- RFLP） methods in 147 gastric cancer patients and 150 healthy controls. Results The frequencies of AA, AC and CC genotype of the promoter - 592 A/C in IL - 10 gene were 45.58% ,42.86% and 11.56% in patients with gastric cancer,while 47.33% ,44.67% and 8.00% in healthy controls. No significant difference was seen in two groups （P 〉0.05）. The allele gene frequency of A and C of the promoter -592 A/C in IL - 10 gene were 67.01% and 32.99% in patients with gastric cancer,while 69.67% and 30.33% in healthy controls. No significant difference was seen in two groups too（P 〉 0.05 ）. Conclusion The polymorphisms of interleukin - 10 gene at the promoter - 592 （A/C） may have nothing to do with the genetic suscepti- bility with the risk of gastric cancer in Ningxia.