HPSEC法测定食用棕榈油中氧化甘油三酯聚合物

Determination of Oxidized Triacylglycerol Polymers in Editable Palm Oil by HPSEC

试验采用制备型快速柱层析-高效体积排阻色谱(HPSEC),先通过制备型快速柱层析技术,将食用棕榈油中的极性组分提取出来。再采用高效体积排阻凝胶色谱对极性组分进行色谱分离分析,以甘油三酯为标准,凡是在甘油三酯之前分离的物质为油脂的氧化甘油三酯聚合物,最后采用峰面积归一化法计算出氧化甘油三酯聚合物在极性组分中的含量,再根据极性组分在全油中的含量,计算出全油中氧化甘油三酯聚合物的含量。结果表明:该方法检出限为0.000 1%,标准品易购得,检测数据稳定、准确,RSD<10%。该方法能满足食用棕榈油的检测要求。

PFC-HPSEC method was applied. Firstly by using preparative flash chromatography （PFC） to extract polar component from edible palm oil; Then by using high performance size exclusion chromatography （HPSEC） to analyze polar components （PC）; Triglycerides as the reference, all the components isoated prior to triglyceride were oxidized triglycerides polymer; And finally, by quantitation of PC in oil and TGP in PC through area normalization method, TGP content in palm oil was calculated. The results showed that this method LOD was 0.000 1%, reference standards were easy to purchased, and test data was stable, accurate and RSD〈 10%. This method was considered suitable for testing requirements of edible palm oil.