摘要
目的:建立同时测定桑叶药材及其炮制品炒桑叶、蜜桑叶中绿原酸、芦丁和异槲皮苷含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent Zorbax SB-C18,流动相为乙腈-0.2%磷酸(梯度洗脱),流速为1.0 ml/min,检测波长为350 nm,柱温为30℃,进样量为10μl。结果:绿原酸、芦丁、异槲皮苷的检测质量浓度线性范围分别为8.20~82.01μg/ml(r=0.999 9)、2.76~27.60μg/ml(r=0.999 9)、4.74~47.39μg/ml(r=0.999 9);精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为98.58%~100.91%(RSD=1.02%,n=6)、99.19%~101.00%(RSD=0.82%,n=6)、98.41%~101.51%(RSD=1.08%,n=6)。结论:该方法操作简便,稳定性、重复性好,可用于桑叶药材及其炮制品中绿原酸、芦丁和异槲皮苷含量的同时测定。
OBJECTIVE:To establish a method for the simultaneous contents determination of chlorogenic acid,rutin and isoquercitrin in Morus alba,fried M. alba and honeyed M. alba. METHODS:HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile-0.2% phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 350 nm,the column temperature was 30 ℃,and injection volume was 10 μl. RESULTS:The linear range was 8.20-82.01μg/ml for chlorogenic acid(r=0.999 9),2.76-27.60 μg/ml for rutin(r=0.999 9)and 4.74-47.39 μg/ml for isoquercitrin(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.58%-100.91%(RSD=1.02%,n=6),99.19%-101.00%(RSD=0.82%,n=6)and 98.41%-101.51%(RSD=1.08%,n=6),respectively. CONCLUSIONS:The method is simple with good stability and reproducibility,and can be used for the simultaneous determination of chlorogenic acid,rutin and isoquercitrin in different processed drugs of M. alba.
出处
《中国药房》
CAS
北大核心
2016年第21期2990-2992,共3页
China Pharmacy