麻黄素对小鼠心肌组织结构和总抗氧化能力、过氧化氢酶活力的影响

Effects of ephedrine on histological structure,activities of total antioxidant capacity and catalase in the myocardial tissue of mice

目的探讨麻黄素对小鼠心肌组织的毒性影响。方法 60只小鼠随机分为麻黄素组和对照组,每组各30只,用递增剂量腹腔连续注射麻黄素溶液(0.0315 g/kg、0.0477 g/kg、0.0564 g/kg)和等量的生理盐水15d(每天两次,每次0.2ml),称量检测小鼠体重和心重的变化,用生物显微技术观察心肌组织结构的变化,用比色法检测血浆肌酸激酶(CK)、肌酸激酶同工酶-MB(CK-MB)和心肌组织总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活力及丙二醛(MDA)含量的变化。结果麻黄素组小鼠体重和心重均低于对照组,小鼠心肌组织出现不同程度的损伤,心肌细胞排列紊乱,闰盘间隙增宽,心肌纤维断裂;麻黄素组小鼠血浆CK、CK-MB活性高于对照组(P<0.01),心肌组织T-AOC和CAT的活性降低(P<0.05或P<0.01),MDA含量则升高(P<0.01)。结论麻黄素影响小鼠心肌组织结构,可能与心肌组织抗氧化酶活性降低和丙二醛含量升高有关。

Objective To study the poisonous effects of ephedrine on the myocardial tissue. Methods Sixty mice were randomly divided into control group and ephedrine group,thirty mice in each group. The mice in ephedrine group were injected with ephedrine at the doses 0. 0315 g / kg,0. 0477 g / kg,0. 0564 g / kg（ × 0. 2ml）,respectively,twice per day for 15 days,and the mice in control group were injected with equal amount of saline. Changes of body weight and heart weight were weighed,the activities of creatine kinase（ CK） 、creatine kinase-MB（ CK-MB） in plasma and the activities of total antioxidant capacity（ T-AOC）,catalase（ CAT） as well as the content of malondialdehyde（ MDA） in myocardial tissue were detected by colorimetry,the changes of the myocardial tissue were observed by optical microscopy. Results Both the body weight and heart weight in ephedrine group were lower than that in the control group. Myocardial tissue of mice appeared different degree of damage after injected ephedrine,myocardial cell arrangement confusion,intercalated disc gap widened,and myocardial fiber breakage were observed. The activities of plasma CK and CK-MB in ephedrine group were significantly higher than that in control group（ P〈0. 01）. The activities of T-AOC and CAT in ephedrine group were significantly lower compared to the control group（ P〈0. 05 or P〈0. 01）. MDA contents in ephedrine group were significantly higher than that in control group（ P〈0. 01）. Conclusion Ephedrine affects the shape and structure of myocardial tissue in mice,which may be correlated with activity decreased of T-AOC and CAT and content increased of MDA.