摘要
目的:研究人胎盘源间充质干细胞(HPMSCs)与Bio-oss骨粉结合后的增殖分化情况,探讨以人胎盘源干细胞(HPMSCs)作为种子细胞,Bio-oss骨粉作为支架材料联合构建组织工程化生物衍生骨的可能性。方法:无菌条件下分离培养人胎盘源间充质干细胞(HPMSCs)并检测其分化能力。将细胞分为单独培养组与复合Biooss骨粉培养组。观察细胞与支架材料结合情况并在细胞形态特点、增殖分化能力方面将两组细胞进行比较。结果:人胎盘源间充质干细胞与Bio-oss骨粉复合培养后,细胞形态、增殖分化能力与对照组人胎盘源间充质干细胞无明显差异。结论:以人胎盘源干细胞(HPMSCs)为种子细胞、Bio-oss骨粉为支架复合培养构建组织工程化衍生骨是可行的。
Objective:To study the proliferation and differentiation of human placenta-derived mesenchymal stem cells(HPMSCs)combined with Bio-oss powder,and investigate the feasibility of constructing tissue engineered bone by HPMSCs as seed cells and the Bio-oss powder as scaffold material.Methods:Human placenta-derived mesenchymal stem cells were dissociated and cultured under sterile conditions and detected for the ability of differentiation.They were divided into two groups,the group of combination of cells and Bio-oss as scaffold material and the group of cells alone.Cell morphology,proliferation and differentiation in the two groups were observed and compared.Results:There was no significant difference between two groups on the cell morphology,proliferation and differentiation ability.Conclusion:It is feasible to utilize HPMSCs as seed cells and Bio-oss as scaffolds to construct tissue engineered bone.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第7期681-684,共4页
Journal of Oral Science Research
基金
吉林省自然科学基金项目(编号:20130101017JC)