天然衍生脱细胞牛心包复合BMP-2转染的BMSCs引导骨组织再生的体外实验研究

Experimental Study on Acellular Bovine Pericardium Combined with BMP-2 Transfected BMSCs in Guided Bone Regeneration in Vitro

目的:研究天然衍生脱细胞牛心包复合BMP-2转染BMSCs引导骨组织再生的可行性和有效性。方法:天然衍生脱细胞牛心包体外复合BMP-2转染BMSCs,SEM评估细胞生长与增殖,MTT法检测牛心包对转染细胞的毒性,ELISA检测BMP-2含量,结晶紫染色法测定BMSCs细胞粘附能力,ALP活性实验检测转染BMSCs成骨性能。结果:SEM发现天然衍生脱细胞牛心包复合BMP-2基因转染的BMSCs后,细胞生长并增殖良好;各组细胞的存活率没有显著性差异;天然衍生脱细胞牛心包增强了BMP-2转染BMSCs的细胞粘附能力和成骨细胞转化能力。结论:天然衍生脱细胞牛心包复合BMP-2基因转染BMSCs后,具有良好的生物和细胞相容性,有望成为一种优良的天然GBR膜材料。

Objective：To study the feasibility of naturally derived acellular bovine pericardium combined with BMP-2gene transfected BMSCs in guided bone tissue regeneration in vitro.Methods：BMP-2transfected BMSCs were seeded on the natural acellular bovine pericardium.The cell growth and proliferation on acellular bovine pericardium were assessed by SEM,the cytotoxicity of membrane on transfected cells was assessed by MTT test,the BMP-2content was measured by ELISA,cell adhesion capacity was detected by crystal violet staining,and osteogenic properties was evaluated by ALP activity.Results：SEM showed that the BMP-2gene transfected BMSCs could adhere to the acellular bovine pericardium and proliferate well.There was no significant difference in the survival rate of cells in each group.The cell adhesion capacity and the ability of BMP-2transfected BMSCs transforming into osteoblasts were enhanced by the acellular bovine pericardium.Conclusion：Natural acellular bovine pericardium had well biological and cellular compatibility,which might be used as a good natural GBR membrane material.