整合素连接激酶和基质金属蛋白酶-9在涎腺腺样囊性癌中的表达及意义

Expression and Significance of Integrin-linked Kinase and Matrix Metalloproteinase-9 in Salivary Adenoid Cystic Carcinoma

目的:探讨整合素连接激酶(ILK)和基质金属蛋白酶-9(MMP-9)在涎腺腺样囊性癌(SACC)中的表达及意义。方法:选取59例SACC患者和42例非肿瘤患者正常涎腺组织,利用qRT-PCR技术检测ILK和MMP-9在SACC组织中表达。结果:SACC组织中ILK mRNA和MMP-9mRNA相对表达量均高于对照组,差异均有统计学意义(P<0.05);ILK和MMP-9在SACC组织中表达均与组织类型、淋巴结转移、TNM分期有关(P<0.05),实体型和管状型SACC组织中ILK mRNA和MMP-9mRNA相对表达量均高于筛状型(P<0.05);Pearson相关分析显示,SACC组织中ILK mRNA相对表达量与MMP-9mRNA相对表达量呈正相关(r=0.586,P=0.000)。结论:ILK和MMP-9在SACC组织中呈高表达,可能共同协同参与了肿瘤细胞侵袭、转移过程。

Objective：To investigate the expression and significance of integrin-linked kinase（ILK）and matrix metalloproteinase-9（MMP-9）in salivary adenoid cystic carcinoma（SACC）.Methods：59cases of SACC specimens and 42 cases of normal salivary gland tissues were collected.The expression of ILK and MMP-9in SACC and normal tissues were detected by using QRT-PCR technology.Results：The mRNA expression levels of ILK and MMP-9were significantly higher in SACC tissues than those in control group（P〈0.05）,and they were positively correlated in SACC tissues（r=0.586,P=0.000）.The expression of ILK and MMP-9in SACC tissues were correlated with disease subtype,lymph node metastasis and TNM stage（P〈0.05）.The mRNA expression levels of ILK and MMP-9in entity and tubular type were obviously higher than sieve-like type（P〈0.05）.Conclusion：Up-regulation of ILK and MMP-9may synergistically promote the invasion and metastasis of tumor cells in SACC.