高迁移率族蛋白B1及其糖基化终产物受体在系统性红斑狼疮患者中的变化及其临床意义

Change and clinical significance of high mobility group protein B1 and its advanced glycation end product receptor in patients with systemic lupus erythematosus

目的探讨高迁移率族蛋白B1(HMGB1)及其糖基化终产物受体(RAGE)在系统性红斑狼疮(SLE)发病中可能的作用。方法采用酶联免疫吸附试验(ELISA)检测52例SLE女性患者(SLE组)及40例体检健康女性(HC组)血浆HMGB1水平;同时采用实时荧光定量PCR(RT-qPCR)检测外周血单核细胞(PBMCs)HMGB1及RAGE mRNA表达水平;并分析SLE患者血浆HMGB1及PBMCs HMGB1、RAGE mRNA水平与临床指标的相关性。结果SLE组患者血浆HMGB1及PBMCs HMGB1mRNA水平高于HC组,差异均有统计学意义(P<0.01);而PBMCs RAGE mRNA水平比较,差异无统计学意义(P>0.05);Spearman相关性分析显示,SLE患者血浆HMGB1水平与抗核抗体滴度、SLE活动性指数(SLEDAI)评分均呈正相关(P<0.05),与其他临床和实验室指标无明显相关性(P>0.05);HMGB1mRNA表达水平与RAGE mRNA表达水平、SLEDAI评分均呈正相关(P<0.01,P<0.05),与其他临床和实验室指标无明显相关性(P>0.05)。结论血浆HMGB1及PBMCs HMGB1mRNA在SLE患者的异常表达提示其可能在SLE的发生、发展中发挥作用,可能参与了SLE的免疫炎症调节。

Objective To investigate the possible role of high mobility group box 1protein（HMGB1）and its advanced glycation end products receptor（RAGE）in the pathogenesis of systemic lupus erythematosus（SLE）.Methods The enzyme-linked immunosorbent assay（ELISA）was used to determine the level of plasma HMGB1 in 52cases of SLE（SLE group）and 40 healthy females undergoing physical examination（HC group）,at the same time real time quantitative polymerase chain reaction（RTqPCR）was employed to detect the expression of HMGB1 and RAGE mRNA in peripheral blood mononuclear cells（PBMCs）.The correlation between plasma HMGB1,PBMCs HMGB1 and RAGE mRNA levels with clinical indicators was analyzed.Results The levels of plasma HMGB1,PBMCs HMGB1 mRNA in the SLE group were significantly higher than those in the HC group,the differences were statistically significant（P〈0.05）,while the level of PBMCs RAGE mRNA had no statistical difference（P〈0.05）;the Spearman correlation analysis showed that the level of plasma HMGB1 was positively correlated with antinuclear antibodies titers and SLEDAI score in the SLE patients（P〈0.01）,while had no obvious correlation with the other clinical and laboratory indicators（P〈0.05）;the HMGB1 mRNA expression level was positively correlated with the RAGE mRNA expression level and SLEDAI scores（P〈0.01,P〈0.05）,and had no obvious correlation with other clinical and laboratory indicators（P〈0.05）.Conclusion The abnormal expression of plasma HMGB1 and PBMCs HMGB1 mRNA in SLE patients prompts that which might be involved in the occurrence and development of SLE,might participate in the immune and inflammatory regulation of SLE.