N-乙酰基半胱氨酸对牙科水门汀生物相容性的影响

The effect of N-acetyl cysteine(NAC) on biocompatibility of dental cements

目的:探讨N-乙酰基半胱氨酸(NAC)对口腔常用水门汀:磷酸锌水门汀(Zpc)、玻璃离子水门汀(FujiⅠ)、树脂改良型玻璃离子水门汀(Fuji plus)、树脂水门汀(G-cem)体外细胞毒性的影响。方法:四种水门汀Zpc、FujiⅠ、Fuji plus、G-cem固化制取标准模型后用培养液制取浸提液,每组各加入不同浓度NAC(0 m M;1 m M;5 m M;10 m M;15m M),再分别与3T3细胞系(小鼠胚胎成纤维细胞)共培养,采用四唑盐显色(MTT)比色分析法,比较不同水门汀加入不同浓度NAC后细胞毒性的变化。结果:四种水门汀Zpc、FujiⅠ、Fuji plus、G-cem具有不同程度的细胞毒性,表现为细胞相对增值率(RGR)的降低,其RGR均值分别为94.72%、80.89%、31.44%、3.89%。而加入不同浓度NAC后各水门汀表现不一:FujiⅠ和Fuji Plus加入1 m M、5 m M NAC时RGR有增高趋势,但无统计学差异,反而在加入15 m M NAC时出现RGR的降低(P<0.05);G-cem和Zpc加入NAC后都相应的降低水门汀细胞毒性,提高细胞相对增殖率,其中5m M和10 m M出现峰值(P<0.05)。结论:不同水门汀其生物相容性差别较大,临床在选择水门汀时应该注意其毒性的影响;加入NAC后可以改善其生物相容性,但是NAC具有浓度依赖性,要注意浓度的选择。NAC减毒机理有待进一步研究。

Objective：To evaluate the potential amelioration of the cytotoxicity of dental cements as follows： Zinc Phosphate Cements（Zpc）, Glass Ionomer Cements（FujiⅠ）,Resin Modified Glass Ionomer（Fuji plus）and Resin Cements（Gcem） by N-acetyl cysteine（NAC） in vitro. Method：Four cements（Zpc,FujiⅠ,Fuji plus,G-cem） were cured according to the manufacturers＇ instructions and extraction mediums were prepared. 3T3-Swiss albino（3T3） cells were exposed to the extraction mediums in the presence of different concentration NAC（0m M,1m M,5m M,10 m M,15 m M）. Cytotoxicity was measured by a modified 3-（4, 5-dimethyl-thiazol-2-yl）-2,5-diphenyl-tetrazoliumbromide（MTT） assay. Result：Four tested cements exhibited varying degrees of cytotoxic effect, and induced the reduction of cell relative growth rate which were 94.72 %, 80.89%, 31.44 %, 3.89 % respectively. The performance of cements were variable in the present of different concentration NAC.The RGR of FujiⅠand Fuji Plus appeared an increasing tendency with 1m M and 5 m M NAC, but there were no statistical difference. Instead, the RGR was lower when added 15 m M NAC（P 0.05）. NAC which were added in the group of G-cem and ZPC exhibited notable detoxification effects and improved the RGR. Meanwhile 5 m M and 10 m M NAC had the best results among different concentration（P 0.05）.Conclusion：The biocompatibility of different dental cements were great different, so pay attention to the toxicity of cements when select the cements in clinical practice. This study demonstrated that NAC can improve the biocompatibility of dental cements which were in a concentration dependent manner. And Further studies are needed to explore the mechanisms.