牛病毒性腹泻病毒TC株的分离鉴定及E0基因的序列分析

Isolation and identification of bovine viral diarrhea virus TC strain and sequence analysis of E0 gene

从新疆塔城地区某牛场采集患病犊牛全血,将RT-PCR方法鉴定为牛病毒性腹泻病毒（BVDV）阳性的样品接种到MDBK细胞,传代至第4代,出现典型病变（CPE）。通过间接免疫荧光试验,证明该分离病毒为BVDV,命名为BVDV-TC。设计引物对E0基因进行扩增和序列分析。所得片段与GenBank上已发表的BVDV毒株核苷酸序列的同源性为69.3%~93.8%,氨基酸同源性为73.6%~97.8%,TC株的遗传距离与VEDEVAC株最接近。BVDV-TC是第一个在新疆地区报道的1b亚型BVDV毒株,为新疆地区BVDV感染的防控提供依据。

Blood samples were collected from cattle suspicious of bovine viral diarrhea virus（ BVDV） in Tacheng areas of Xinjiang and identified by RT-PCR amplication. Positive samples were inoculated to MDBK cells,and the cells presented classical and regular cytopathic effects（ CPE）. The virus was identified as BVDV（ designated as BVDV-TC） by specific indirect immunofluorescent assay. A pair of specific primers was designed based on E0 nucleotide sequence of BVDV published in Gen Bank,and E0 gene of BVDV- TC was amplified by PCR. Compared with E0 sequences of the other BVDV strains published in Gen Bank,nucleotide and amino acid sequence homology was69. 3% ～ 93. 8% and 73. 6% ～ 97. 8%,respectively. The genetic distance of TC strain is close to VEDEVAC. BVDV-TC belongs to 1b subtype firstly reported in Xinjiang,which provides the basis for the prevention and control of BVDV infection in Xinjiang area.