摘要
为了探讨黑果腺肋花楸花色苷对紫外辐射所致人皮肤成纤维细胞氧化损伤的保护作用,将体外培养的人皮肤成纤维细胞分为对照组、辐射组和辐射给药组。采用MTT法检测不同浓度花色苷添加量对细胞增殖的保护作用,以选择最优添加浓度。采用化学荧光法检测细胞活性氧(ROS)含量,ELISA法检测细胞MMP-1分泌水平。结果表明:UVA辐照剂量为10 J/cm2条件下,与辐射组相比,MTT法显示花色苷添加组浓度为125μg/m L对损伤细胞增殖保护作用有极显著的提高(P<0.01),同时,125μg/m L花色苷添加组能够显著降低辐射损伤后细胞ROS含量以及MMP-1分泌水平(P<0.01)。由此可见,适宜浓度的黑果腺肋花楸花色苷能够降低细胞ROS含量及MMP-1分泌水平,从而对辐射损的细胞起到一定的保护作用。
In order to study the protective effect of Aronia melanocarpa anthoeaynins against UV-induced damages in hu- man skin fibroblast, the cells were divided into control group, UVA radiation group and anthocyanidin group. The protec- tive effects of different concentrations of anthoeyanius on cell proliferation were investigated using MTT method. The re- active oxygen species （ROS）was evaluated by fluorescence and chemiluminescence, the MMP-1 protein in cells were de- tected by ELISA assay. The MTT analysis result showed that 125 μg/mL of anthocyanin had an excellent protective effect on cell proliferation. The contents of ROS and MMP-1 in 125 μ,/mL anthocaynin group were significant lower than those in UVA radiation group （ P 〈 0.01 ）. Hence, it was concluded that A. melanocarpa anthocaynins was able to reduce UV-indueed oxidative damage and played an anti-UV radiation role in human skin fibroblast.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第7期1088-1092,共5页
Natural Product Research and Development
基金
河北省教育厅重点项目(ZD2015052)
