响应面优化转谷氨酰胺酶催化燕麦麸皮球蛋白糖基化接枝条件

Optimization of Transglutaminase-catalyzed Glycation Conditions of Oat Bran Globulin by Response Surface Methodology

以燕麦麸皮球蛋白为主要原料,采用单因素和响应面法优化接枝条件,利用转谷氨酰胺酶(TG)修饰制备具有高接枝度的糖基化燕麦麸皮球蛋白。实验结果表明,当氨基糖与蛋白浓度比为0.94∶1、TG酶添加量57.59 U/g、p H值7.65、时间4 h、温度45℃时,糖基化燕麦麸皮球蛋白的接枝度为45.6774%,与响应面方法预测结果相似。此时蛋白体系中的游离氨基为0.3790 mol/kg,修饰蛋白交联体系较好,适于燕麦麸皮蛋白深加工产品开发应用。

The glycosylated oat bran globulin with higher grafting degree was produced by the catalysis of transglutami- nase（TG）. Single factor design and response surface methodology（RSM） were used to optimize the grafting condition. The results indicated that the optimal conditions were ratio of amino sugars and globulin concentration of 0. 94： 1, TG adding amount of 57.59 U/g, pH 7.65,4 h and 45 ℃. Under these conditions, the grafting degree of glycosylated oat bran globulin was 45. 6774% ,which was consistent with the predicated value. This modified protein system with 0.379 mol/kg of free amino further confirmed that the transglutaminase-catalyzed oat bran globulin presented better grafting system than the original one. The results of this study provided a potential further application for the oat bran protein in development of new product.