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维生素D对羊膜腔注射脂多糖导致的新生鼠肺泡化阻滞的作用 被引量:5

Effects of Vitamin D on intra-amniotic lipopolysaccharides-induced alveolarization arrest in neonatal rats
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摘要 目的探讨维生素D(Vit D)对宫内绒毛膜羊膜炎诱发的肺泡化阻滞的改善作用及可能的机制。方法通过孕鼠羊膜腔注射脂多糖(LPS)建立新生鼠支气管肺发育不良(BPD)模型。分为Saline组、LPS+Saline组、LPS+Vit D(L)组和LPS+Vit D(H)组。Vit D干预的2组新生鼠出生后每日腹腔注射0.5和3 ng/g的1,25(OH)_2D_3,Saline组和LPS+Saline组注射等量生理盐水。取第1、3、7日的新生鼠肺组织,通过苏木精-伊红(H-E)染色观察病理学改变。采用real-time PCR检测IL-1β和IFN-γmRNA表达。结果不同剂量的Vit D均显著提高新生鼠出生率(均P=0.003)。出生后7 d,Vit D干预的2组较LPS+Saline组肺泡计数显著增多(P=0.001,P=0.000),平均内衬间隔显著减少(均P=0.000),且Vit D干预的2组IL-1β和IFN-γmRNA表达显著低于LPS+Saline组(均P=0.000)。结论予以Vit D可降低新生鼠肺部IL-1β和IFN-γ表达,减轻BPD病理学改变。 Objective To investigate the role of Vitamin D (VitD) on amelioration of chorioamnionitis-induced alveolarization arrest and possible mechanisms. Methods A neonatal rat bronchopulmonary dysplasia (BPD) model was constructed by intra-amniotic injection of lipopolysaccharides (LPS) in pregnant rats. Rats were randomly assigned to the Saline group, LPS + Saline group, LPS + VitD (L) group, and LPS + VitD (H) group. Neonatal rats in the LPS +VitD (L) group and the LPS +VitD (H) group were intraperitoneally injected with 0.5 and 3 ng/g 1, 25(OH)+ Da respectively once a day for 7 d. Meanwhile, neonatal rats in the Saline group and the LPS + Saline group were intraperitoneally injected with the same volume of normal saline. The pulmonary tissues of neonatal rats were harvested 1, 3, and 7 d after the final injection and were stained by H-E staining for observing pathological changes. The mRNA expressions of IL-1β and IFN-y were detected using real-time PCR. Results Different concentrations of VitD could improve the birth rate of neonatal rats (P = 0. 003). The LPS +VitD (L) group and the LPS +VitD (H) group had significantly higher alveolar counts (P=0. 001, P=0. 000), remarkably decreased mean liner intercept (P =0. 000), and significantly decreased mRNA expressions of IL-1β and IFN-y as compared with the LPS + Saline group 7 d after birth (P=0. 000). Conclusion Administration of VitD can significantly decrease the expressions of IL-1β and IFN-7 in lungs of neonatal rats and alleviate the pathological changes of BPD.
出处 《上海交通大学学报(医学版)》 CAS CSCD 北大核心 2016年第7期969-973,979,共6页 Journal of Shanghai Jiao tong University:Medical Science
基金 国家自然科学基金(81270729)~~
关键词 维生素D 支气管肺发育不良 炎症 Vitamin D bronchopulmonary dysplasia inflammation
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参考文献19

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