摘要
A reconstruction method guided by early-photon fluorescence yield tomography is proposed for time-domain fluorescence lifetime tomography （FLT） in this study. The method employs the early-arriving photons to reconstruct a fluorescence yield map, which is utilized as a priori information to reconstruct the FLT via all the photons along the temporal-point spread functions. Phantom experiments demonstrate that, compared with the method using all the photons for reconstruction of fluorescence yield and lifetime maps, the proposed method can achieve higher spatial resolution and reduced crosstalk between different targets without sacrificing the quantification accuracy of lifetime and contrast between heterogeneous targets.
A reconstruction method guided by early-photon fluorescence yield tomography is proposed for time-domain fluorescence lifetime tomography （FLT） in this study. The method employs the early-arriving photons to reconstruct a fluorescence yield map, which is utilized as a priori information to reconstruct the FLT via all the photons along the temporal-point spread functions. Phantom experiments demonstrate that, compared with the method using all the photons for reconstruction of fluorescence yield and lifetime maps, the proposed method can achieve higher spatial resolution and reduced crosstalk between different targets without sacrificing the quantification accuracy of lifetime and contrast between heterogeneous targets.
基金
supported by the National Natural Science Foundation of China(Nos.81227901,81271617,61322101,and 61361160418)
the National Major Scientific Instrument and Equipment Development Project of China(No.2011YQ030114)
