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雷帕霉素单独及联合化疗药物应用对胃癌细胞生长的影响 被引量：3

Effect of rapamycin combination chemotherapy drugs on inhibition of gastric cancer cells
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摘要 目的:研究mTOR抑制剂雷帕霉素对SGC-7901胃癌细胞增殖的影响及其可能的机制,并探讨其与传统化疗药物的联合作用。方法:体外培养胃癌SGC-7901细胞,取对数生长期的细胞进行试验,用不同浓度的mTOR抑制剂雷帕霉素以及长春新碱分别作用于胃癌SGC-7901细胞24、48、72小时,用MTT法检测雷帕霉素单独以及联合化疗药物对其生长的影响。用流式细胞术检测mTOR抑制剂雷帕霉素、长春新碱单独以及联合应用对胃癌SGC-7901细胞周期的影响。结果:MTT法结果显示:雷帕霉素单独能够显著抑制胃癌SGC-7901细胞的增殖,其作用随着药物浓度以及作用时间的增加而增加,呈现时间-剂量效应关系;与化疗药物长春新碱联合应用其抑制效应明显增强。流式细胞术检测结果显示:雷帕霉素、长春新碱单独作用胃癌细胞将细胞周期主要阻滞在G_0/G_1期,而联合给药时G_0/G_1期比例增加。结论:mTOR抑制剂雷帕霉素能够显著抑制胃癌SGC-7901细胞的增殖,其机制可能与通过阻滞细胞周期在G_0/G_1期相关;并且雷帕霉素可提高传统化疗药物长春新碱对胃癌细胞的敏感性。_ Objective：To investigate the influence of rapamycin on human gastric cancer SGC -7901 cells prolif-eration and its mechanism.Methods：Gastric cancer cells SGC -7901 were cultured and treated with rapamycin,vin-cristine and combination of the two.Then the inhibitory effects of the three medications on the growth of the SGC -7901 cells were determined by MTT,and their cell cycle were detected by flow cytometry.Results：Compared with the control group,the three medications all significantly inhibited the proliferation of SGC -7901 cells,and the combined method enhanced the effect.Flow cytometry showed that rapamycin and vincristine blocked the cell cycle mainly in the G0 /G1 stage,the combined medication enhanced this effect.Conclusion：Rapamycin can inhibit the proliferation of SGC -7901 cells and enhance the chemosensitivity of gastric cancer.
作者 王广胜 朱晨宇 杨振华 刘超 朱耀明 商远方
机构地区 三峡大学第一临床医学院
出处 《现代肿瘤医学》 CAS 2016年第13期2019-2022,共4页 Journal of Modern Oncology
关键词 雷帕霉素 哺乳动物雷帕霉素靶蛋白 胃癌 化疗敏感性 rapamycin, mammals rapamycin target protein, gastric cancer, ehemosensitivity
分类号 R735.2 [医药卫生—肿瘤]
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现代肿瘤医学
2016年 第13期

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