RASSF4的异常表达在非小细胞肺癌中的意义

The significance of RASSF4 abnormal expression in human non-small cell lung cancer

目的:探讨RASSF4在非小细胞肺癌中的表达模式和意义。方法:应用免疫组化及Western Blot方法,检测并分析89例非小细胞肺癌样本中RASSF4的表达情况及其与临床病理因素的相关性。结果:在非小细胞肺癌组织中,41.57%(37/89)出现RASSF4的表达下调,并且其异常表达与患者的高TNM分期(P=0.005)、淋巴结转移(P=0.024)及患者不良预后(P=0.018)相关。在肺癌细胞系中,我们在部分细胞系(57.14%,4/7)中发现了RASSF4的异常表达。结论:RASSF4蛋白的弱表达可能是判断非小细胞肺癌恶性程度的重要标志之一。

Objective：To explored the expression pattern and significance of RASSF4 in NSCLC specimens. Methods：Samples were tested by immunohistochemistry and Western Blot.Results：RASSF4 was downregulated in 41.57%（37 /89）NSCLC tissues,and the abnormal expression of RASSF4 was significantly associated with advanced TNMstage（P =0.005）,positive nodal status（P =0.024）,and poor prognosis（P =0.018）.We examined RASSF4 protein expression in normal lung epithelial cell line and lung cancer lines,and found that RASSF4 expression was downregulated in four of seven（57.14%,4 /7）lung cancer cell lines compared with normal bronchial epithelial cells. Conclusion：Abnormal Pygo2 protein expression may be a marker for advanced NSCLC.