miR-210在肝癌中的异常表达及其与预后的相关性

The expression and prognostic association of miR-210 in hepatocellular carcinoma

目的:检测miR-210在肝癌组织中的表达,探讨其在肝癌中的表达与术后患者预后的关系。方法:收集120例患者术后新鲜肝癌组织,荧光PCR技术检测miR-210在肝癌组织中的表达。检验miR-210表达水平与各临床指标的关系,K-M曲线描述miR-210的表达与预后的关系,Cox回归分析进行多因素分析。结果:miR-210在肝癌组织的表达要比癌旁高(P<0.001),K-M曲线分析发现miR-210高表达预示着较差的预后(P<0.001),Cox回归分析中发现miR-210在单因素分析中P=0.003,在多因素分析中P=0.002。结论:miR-210在肝癌组织中表达高于癌旁组织,miR-210可以作为独立的分子标志提示肝癌术后患者的预后。

Objective：To detect the expression of miR -210 in hepatocellular carcinoma,and investigate their re-lationships with the progression of hepatocellular carcinoma.Methods：All 120 hepatocellular carcinoma samples and peri -cancer tissues were analyzed using real -time quantitative reverse transcriptase polymerase chain reaction.Re-sults：Expression of miR -210 in cancer was higher than the peri -cancer（P〈0.001）.Expression of miR -210 in cancer tissues was significantly associated with TNMgrade （P〈0.05）and tumor size（P〈0.05）.In addition,in-creased miR -210 levels were also associated with overall survival （OS）rates compared to the low expression （P〈0.001 ）,as calculated by Kaplan -Meier survival and Cox regression analyses.Furthermore,subgroup analyses showed that miR -210 expression was significantly associated with OS of HCC patients.Conclusion：miR -210 is highly expressed in HCC and a predict marker of prognosis in HCC patients.