RNA干扰下调ADAR1基因表达对肝癌细胞增殖能力的影响

The impact of RNA interference-induced ADAR1 down-regulation on cell proliferation of liver cancer

目的观察RNA干扰下调ADAR1基因表达对肝癌细胞增殖能力的影响。方法用小分子干扰RNA（siRNA）转染肝癌细胞SMMC-7721，RT-PCR法检测ADAR1mRNA表达，Westernblotting法测定ADAR1蛋白，MTr比色法检测SMMC-7721细胞增殖。结果细胞转染24、48、72小时后，实验组ADAR1mRNA相对表达量分别为0．612±0．086、0．264±0．018、0．156±0．063，对照组分别为1．032±0．107、0．898±0．092、0．968±0．074，差异具有统计学意义（P〈0．05）；对照组与空白组ADAR1mRNA表达差异无统计学意义（P〉0．05）。实验组ADAR1蛋白相对表达量分别为0．684±0．079、0．324±0．042、0．145±0．058，对照组分别为1．002±0．092、0．917±0．068、0．972±0．073，差异均有统计学意义（P〈0．05）；对照组与空白组ADAR1mRNA表达差异无统计学意义（P〉0．05）。细胞转染后SMMC．7721细胞增殖活性明显下降（P〈0．05）。结论ADAR1基因特异性siRNA干扰后，ADAR1mRNA及蛋白相对表达水平显著下降，SMMC-7721细胞的增殖能力亦明显下降。

Objective To observe the impact of RNA interference-induced ADAR1 down-regulation on cell proliferation of liver cancer. Methods Small interfering RNA （siRNA） was transfected into liver cancer cell line SMMC-7721, ADAR1 expression was detected by RT-PCR and Western blotting. Cell prolif- eration was determined by methyl thiazol tetrazolium （MTF） assay. Results After transfection for 24, 48, and 72 h, ADAR1 mRNA expression was 0.612±0.086, 0.264±0.018, 0. 156±0.063 in experimental group and 1. 032±0. 107, 0. 898±0. 092, 0. 968±0. 074 in control group, respectively. Experimental group had significantly lower ADAR1 mRNA than the other groups （P 〈 0.05 ）, and there was no statistically significant difference between control and blank group （P 〉 0. 05）. ADAR1 protein relative expression was 0. 684±0. 079, 0. 324 ±0. 042, 0. 145±0. 058 in experimental group and 1. 002±0. 092, 0. 917± 0. 068, 0. 972±0.073 in control group, respectively, which was statistically significant （ P 〈 0.05 ）. After transfeetion with siRNA, the proliferation ability of SMMC-7721 cells was enormously inhibited （ P 〈 0.05 ）. Conclusion ADAR1 mRNA and protein level could be significantly decreased by specific RNA interfe- rence, and cell proliferation in SMMC-7721 ceils were also greatly inhibited.