北五味子“红珍珠”组织培养研究

Tissue Culture Research of Schisandra chinensis ‘Red Pearl'

[目的]研究适合北五味子"红珍珠"组织培养的方法。[方法]以北五味子"红珍珠"为试材,研究不同外植体、激素对其组织培养诱导、分化和生根的影响。[结果]以春季嫩枝为外植体,分化培养基为1/2MS+6-BA 2.0 mg/L+NAA 0.01 mg/L+ZT0.1 mg/L,以1/2MS+IAA 1.0 mg/L+IBA 2.0 mg/L生根效果较好,有利于北五味子"红珍珠"根系的生长。[结论]筛选出北五味子"红珍珠"适宜的组织培养配方,为其大规模工厂化育苗提供技术支撑。

Objective] The aim was to obtain the suitable tissue culture method for Schisandra chinensis‘Red pearl’ .[ Method] The effects of different explants and hormones on the induction, differentiation and rooting of tissue culture were studied.[ Result] With spring shoots as explants, the differentiation culture medium was 1/2MS ＋6-BA 2.0 mg/L ＋NAA 0.01 mg/L ＋ZT 0.1 mg/L.1/2MS＋IAA 1.0 mg/L＋IBA 2.0 mg/L had better rooting effect, which was conducive to the growth of root system of Schisandra chinensis‘Red pearl’.[Conclu-sion] The suitable tissue culture formula of Schisandra chinensis‘Red pearl’ is selected, which can provide the technical support for large-scale breeding.