黄尾鲴不同组织中LDH同工酶的研究

Study on LDH Isozyme in Different Tissues of Xenocypris davidi

[目的]研究黄尾鲴不同组织的LDH同工酶。[方法]采用垂直板聚丙烯酰胺凝胶电泳技术和特异性染色方法对黄尾鲴的肝、肾、眼、心4种组织的LDH同工酶进行分析。[结果]酶谱的表达具有明显的组织特异性。酶带由3个基因座位(LDH-A、LDH-B、LDHC)编码,在4种组织中共检测到14条带,其中,心和肾7条带,眼6条带,肝14条带。在PAGE胶趋向阴极侧检测到6条肝脏组织所特有的LDH-C基因编码带。在各组织LDH酶带表达活性上,心中LDH-2、LDH-3和LDH-5表达占优势,肾中LDH-2、LDH-7和LDH-3表达占优势,肝中LDH-13、LDH-9和LDH-12表达占优势,眼中LDH-5、LDH-2和LDH-7表达占优势。[结论]该研究为丰富黄尾鲴遗传学基础研究、遗传育种种质标准的建立和种群生化遗传结构分析提供科学依据。

ObjectiveThe aim was to study LDH isozyme in different tissues of Xenocypris davidi.[Method] By using vertical polyacrylamide gel electrophoresis and specific dye measures, LDH isozyme in 4 tissues （liver, kidney, eye and heart） of Xenocypris davidi was studied.[Re-sult] The results showed that the electrophoretogram of LDH isozyme patterns exhibited remarkable tissue specificities .14 LDH isozyme bands en-coded by LDH-A, LDH-B, LDH-C loci were detected from all the 4 tissues.7 bands were found in heart and kidney, 6 bands were found in eye, and 14 bands were found in liver.6 specific bands encoded by LDH-C were detected on the cathode side of PAGE hectograph within liver. According to activity of LDH isozyme in tissues, LDH-2, LDH-3 and LDH-5 were preponderantly expressing in heart, LDH-2, LDH-7 and LDH-3 were preponderantly expressing in kidney, LDH-13, LDH-9 and LDH-12 were preponderantly expressing in liver, and LDH-5, LDH-2 and LDH-7 were preponderantly expressing in eye.[Conclusion] The study provides a scientific basis for enrichment of genetic ba-sis research, establishment of genetic breeding germplasm standard and analysis of population biochemical genetic structure .