氧化锌@石墨烯纳米复合材料的抑菌性能及其细胞毒性

Antibacterial activity and cytotoxicity of ZnO@graphene nanocomposites

以氧化石墨和乙酸锌为原料,通过水热反应成功制备了氧化锌@石墨烯纳米复合材料,采用X射线衍射(XRD)、红外吸收光谱(FTIR)和透射电子显微镜(TEM)对制得的复合材料进行了表征.以大肠杆菌(E.coli)为实验菌种,对复合材料的抑菌性能进行了测试;并选用小鼠成纤维细胞L-929评价了材料的细胞毒性.结果表明,纳米氧化锌颗粒均匀地负载在石墨烯片层上,形貌均一,平均粒径为12 nm左右.复合材料在60μg·m L-1时可以完全抑制大肠杆菌的生长,是一种效果显著的新型抑菌材料.L-929细胞毒性测试表明复合材料的生物毒性比较缓和,氧化锌@石墨烯纳米复合材料可以作为一种安全高效的无机抑菌材料使用.

ZnO @ graphene nanocomposite were prepared by hydrothermal method,using graphite oxide（ GO） and zinc acetate as raw materials. X-ray diffraction（ XRD）,Fourier transform infrared spectroscopy（ FTIR） and transmission electron microscope（ TEM） were used to characterize the prepared nano-composite. The antibacterial activity of ZnO @ graphene nano-composites was tested using E.coli as mode strain. Moreover,cytotoxicity was assessed using L-929 mouse fibroblast cells.The results of characterization indicated that zinc oxide nanoparticles were uniformly dispersed on graphene surface with particle size around 12 nm. The minimal inhibitory concentration of ZnO @graphene against E.coli was 60 μg·mL^-1,suggesting that the composite was an outstanding antibacterial material. Additionally,the toxicity tests demorstrated that the prepared nano-composite posed no significant toxicity on L-929 cells. Therefore,ZnO@ graphene nano-composites can be used as a new type of antibacterial material.