运动功能饮料中合成着色剂测定及全自动固相萃取仪的应用

Determination of Synthetic Food Colorants in Sports Drink and Application of Automated Solid Phase Extraction

摘要：建立了应用全自动固相萃取仪进行样品净化，高效液相色谱DAD检测器测定运动功能饮料中6种合成着色剂的方法。样品用去离子水稀释，经过固相萃取小柱净化后，经高效液相色谱仪DAD检测器测定。结果表明：6种合成着色剂3个水平（0．001、0．005、0．050g／kg）加标回收率为94．5％～100．9％，相对标准偏差为0．32％～1．02％（n=6），检出限分别为柠檬黄0．05mg／kg、日落黄0．1mg／kg、胭脂红0．1mg／kg、诱惑红0．1mg／kg、苋菜红0．1mg／kg、亮蓝0．1mg／kg。此方法具有简单、快速、准确等优点，适用于运动饮料中柠檬黄、日落黄、胭脂红、诱惑红、苋菜红和亮蓝的同时测定。

Samples was diluted by water, and purified by solid phase extraction column, then determined by HPLC with DAD detector. The recoveries for synthetic food colorants standard spiked in the range of 0.001 to 0.05 g/kg were between 94.5% to 100.9% with a relative standard deviation（RSD） being between 0.32% to 1.02%（n=6）. The detection limit was Lemon yellow： 0.1 mg/kg, Sunset yellow： 0.1 mg/kg, Carmine： 0.1 mg/kg,Allura red： 0.1mg/kg, Amaranth： 0.1 mg/kg, Brilliant blue： 0.1 mg/kg. This method is suitable for the determination of synthetic food colorants in sports drink.