QKI-5在三阴乳腺癌组织中的表达及其意义

Expression and significance of QKI-5 in triple negative breast cancer

目的 :利用免疫组织化学方法检测RNA结合蛋白quaking 5(QKI-5)在人三阴乳腺癌组织中的表达,探讨QKI-5在乳腺癌转移过程中的作用。方法:采用组织芯片技术,检测30对人三阴乳腺癌组织及其对应癌旁组织中的QKI-5蛋白水平表达情况,并对表达情况与临床病理参数间的关系进行分析。构建能稳定过表达QKI-5的细胞株MDA-MB-231和稳定敲减QKI-5表达的细胞株4T1,并对其迁移功能进行测定。结果 :肿瘤组织中的QKI-5表达率(13.3%)较对应癌旁组织(53.3%)低(χ2=10.8,P<0.05);且肿瘤组织中QKI-5的阴性表达与患者的淋巴结转移及肿瘤直径有关(P=0.025,P<0.05;P=0.015,P<0.05);体外迁移实验结果显示,过表达QKI-5的MDA-MB-231细胞的迁移能力下降(t=3.0,P<0.05),而敲减QKI-5表达的4T1细胞的迁移能力提升(F=22.9,P<0.05)。结论:QKI-5在人三阴乳腺癌组织中低表达,而其低表达可能是促进三阴乳腺癌淋巴结转移的重要原因。

Objective To detect the expression of QKI-5 in triple negative breast cancer by immunohistoehemistry, and to investigate the significance of QKI-5 in regulation of metastasis of breast cancer. Methods QKI-5 expression was detected by tissue microarray in 30 pairs of invasive breast ductal carcinoma sample and adjacent non-caneeroustissue. Correlation of QKI-5 expression with clinicopathologic features of breast cancer were analyzed. Breast cancer cell line MDA-MB-231 stably over-expressing QKI-5 was established by using lentiviral vectors, and a 4T1 cell line with knockeddown QKI-5 expression was served as control. The effects of QKI-5 expression on 4T1 cells and MDA-MB-231 cells were assessed by migration assay. Results Expression rate of QKI-5 in invasive breast ductal carcinoma samples was lower than that in adjacent non-cancerous tissue（χ^2=10.8,P〈0.05）. Negative expression of QKI-5 was closely correlated with lymph node metastasis and tumor size （P=0.025 ,P〈0.05 ;P=0.015,P〈0.05）. Migration ability of MDA-MB-231 cells was significantly inhibited in vitro（t=3.0 ,P〈0.05）,while knock-down of QKI-5 in 4T1 cells could promote cell migration ability in vitro （F= 22.9,P〈0.05）. Conclusions QKI-5 is significantly suppressed in tissue of triple negative breast cancer, and the low expression of QKI-5 may contribute to the lymph node metastasis of breast cancer.