摘要
目的检测三叉神经痛(TN)模型大鼠的三叉神经节(TG)神经元中NPR-A受体mRNA表达量的变化。方法采用眶下神经缩窄术和经眶下孔注射肿瘤坏死因子α(TNF-α)将雄性SD大鼠随机分成6组,即对照组1(假手术组)、对照组2(生理盐水注射组)、眶下神经缩窄模型组(ION-CCI组)术后1周和2周以及经眶下孔注射TNF-α组(TNF-α组)注射后1周和2周。Q-PCR法检测NPR-A受体mRNA的表达。结果每组大鼠TG均表达NPR-A受体mRNA。其中ION-CCI组术后2周以及TNF-α组注射后1周和2周TG内NPR-A受体mRNA表达含量均显著高于相应对照组(n=4,P<0.05)。结论大鼠TG表达的NPR-A受体可能与大鼠TN及炎性痛的发生和发展相关。
Objective To investigate the expression of NPR-A receptor on mouse trigeminal ganglions of trigemiual neuralgia model. Methods Male SD rats were randomly assigned into 6 groups : control group 1 ( sham group ) , control group 2 ( infraorbital foramen inject normal saline group), chronic constriction injury of the infraorbital nerve guoup(ION-CCI group) 1 week and 2 weeks and infraorbital foramen inject TNF-α group(TNF-α group) 1 week and 2 weeks. The expressions of NPR-A mRNA were detected by Q-PCR. Results The expression of NPR-A was detected in all groups. And the expressions of NPR-A in the ION-CCI group 2 weeks and TNF-α group 1 week and 2 weeks were higher than that in the corresponding control group. Conclusion NPR-A receptor on mouse trigeminal ganglions may be involved in the trigeminal neuralgia and inflmmatory pain.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第8期1097-1100,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81271162)
安徽省高等学校省级优秀青年人才基金项目(编号:2012SQRL070)
安徽省高校省级优秀青年人才基金重点项目(编号:2013SQRL146ZD)
关键词
三叉神经节
慢性压迫性损伤
三叉神经痛
trigeminal ganglion
chronic constriction injury
trigeminal neuralgia