三叉神经痛模型大鼠三叉神经节神经元中NPR-A表达量的变化

Variation of NPR-A receptor expression on mouse trigeminal ganglion neurons of trigeminal neuralgia model

目的检测三叉神经痛(TN)模型大鼠的三叉神经节(TG)神经元中NPR-A受体mRNA表达量的变化。方法采用眶下神经缩窄术和经眶下孔注射肿瘤坏死因子α(TNF-α)将雄性SD大鼠随机分成6组,即对照组1(假手术组)、对照组2(生理盐水注射组)、眶下神经缩窄模型组(ION-CCI组)术后1周和2周以及经眶下孔注射TNF-α组(TNF-α组)注射后1周和2周。Q-PCR法检测NPR-A受体mRNA的表达。结果每组大鼠TG均表达NPR-A受体mRNA。其中ION-CCI组术后2周以及TNF-α组注射后1周和2周TG内NPR-A受体mRNA表达含量均显著高于相应对照组(n=4,P<0.05)。结论大鼠TG表达的NPR-A受体可能与大鼠TN及炎性痛的发生和发展相关。

Objective To investigate the expression of NPR-A receptor on mouse trigeminal ganglions of trigemiual neuralgia model. Methods Male SD rats were randomly assigned into 6 groups ： control group 1 （ sham group ） , control group 2 （ infraorbital foramen inject normal saline group）, chronic constriction injury of the infraorbital nerve guoup（ION-CCI group） 1 week and 2 weeks and infraorbital foramen inject TNF-α group（TNF-α group） 1 week and 2 weeks. The expressions of NPR-A mRNA were detected by Q-PCR. Results The expression of NPR-A was detected in all groups. And the expressions of NPR-A in the ION-CCI group 2 weeks and TNF-α group 1 week and 2 weeks were higher than that in the corresponding control group. Conclusion NPR-A receptor on mouse trigeminal ganglions may be involved in the trigeminal neuralgia and inflmmatory pain.