柠檬醛调控突变型p53基因和AIF基因表达诱导急性早幼粒细胞白血病细胞株NB4凋亡

Mechanisms of apoptosis in NB4 cells induced by citral via regulation of mtp53 gene and AIF gene

目的探讨柠檬醛诱导急性早幼粒细胞白血病细胞株NB4凋亡的分子机制。方法用不同浓度的柠檬醛处理NB4细胞48 h,通过实时荧光定量PCR(q PCR)法检测突变型p53(mtp53)基因和凋亡诱导因子AIF基因的mRNA表达变化。结果柠檬醛实验组与空白对照组比较,mtp53基因表达量减低(P<0.05);柠檬醛实验组与空白对照组比较,AIF基因表达量增加(P<0.05);乙醇对照组与空白对照组比较,mtp53和AIF的mRNA表达量无明显差异;随着柠檬醛浓度(5、10、20 mg/L)的递增,mtp53 mRNA表达量逐渐下降,AIF mRNA的表达量逐渐上升,mtp53、AIF在不同浓度柠檬醛实验组表达量差异有统计学意义(P<0.05)。结论柠檬醛通过下调NB4细胞mtp53的表达,上调AIF的表达诱导NB4细胞凋亡。

Objective To investigate the mechanism of the apoptosis induced by citral in the acute promyelocytie leukemia cell line NB4 . Methods NB4 cells were treated with citral for 48 h at the various concentration , and then the mRNA expression of mutation p53 （ mtp53 ） and AIF was detected by method of qPCR. Results The expression of mtp53 gene decreased when the critral groups compared with the control group, the difference between them was statistically significant （P 〈 0.05 ） ;the expression of AIF gene was increased when the critral group compared with the control group, the difference between them was statistically significant （ P 〈 0.05 ） ; ethanol group compared with the control group, mRNA expressions of mtp53 and AIF had no significant difference. With eitral concentration （5,10,20 mg/L） increasing, the level of expression of mtp53 mRNA reduced, while the level of expression of AIF mRNA gradually increased, both mtp53 and AIF expression in different concentrations of citral group differences were statistically significant （P 〈 0. 05 ）. Conclusion Citral can induce apoptosis in NB4 cells by downregulating the expression of mtp53 and upregulating the expression of AIF.