WPC纳米纤维聚合物体外消化性能的研究

Digestion properties of WPC Namo- fibril aggregates in vitro

为了研究纳米纤维聚合物的体外消化性能,本文对不同加热时间下纳米纤维聚合物和常规聚合物性能与指标进行测定,并探讨了胃蛋白酶和胰蛋白酶对2种不同结构聚合物的体外消化作用。结果表明,纳米纤维聚合物的表面疏水性和游离巯基含量比常规聚合物高。在相对较低的酶浓度(E/S为1/30)条件下,与常规聚合物对比,纳米纤维聚合物的水解度较高;但是,增加酶浓度(E/S为1/3)导致的水解度提高幅度远不如WPC常规聚合物,尤其对胰蛋白酶而言,几乎没有任何提高,并且纳米纤维聚合物的水解度要低于常规聚合物的水解度,但对胃蛋白酶而言,酶的添加量的增加依然使纳米纤维聚合物的水解度高于常规聚合物的水解度。对于WPC纳米纤维聚合物形成的3个时期而言,成核期有利于胃蛋白酶的水解,稳定期有利于胰蛋白酶的水解。可以得出结论:体外消化过程中,纳米纤维聚合物更容易被水解。

In order to study the properties of in vitro digestion of namo -fibril aggregates, the digestion of namo - fibril aggregates and normal aggregates for pepsin and trypsin in vitro was investigated in this paper, and the effect of heating time on the digestion of them were also discussed. The results showed that the surface hydrophobicity and the contents of the free sulfhydyl group of namo -fibrils aggregates were higher than those of the normal WPC aggregates.Compared with normal WPC aggregates, the namo -fibril aggregates had higher degree of hydrolysis at low enzyme concentrate（ E/S 1/30）.However, the improvement degree of hydrolysis was much less than normal WPC aggregates,especially, no any change was observed with the increase content of the trypsin,and the degree of hydrolysis of the namo -fibrils aggregates was lower than the degree of hydrolysis of normal aggregates, but for the pepsin,the degree of hydrolysis of the namo -fibrils aggregate was higher than that of normal aggregates as the increasing of the amount of enzyme. Nucleation of namo -fibrils was good for hydrolysis of pepsin,while termination of namo -fibrils was good for trypsin hydrolysis during WPC fibril formation three steps. It can be concluded, in vitro digestion process, the namo - fibril aggregates were more easily hydrolyzed.