口腔鳞状细胞癌中PLK1蛋白表达与中心体扩增

PLK1 Protein Expression and Centrosome Amplification in Oral Squamous Cell Carcinoma

目的:探讨口腔鳞状细胞癌(Oral Squamous Cell Carcinoma,OSCC)中保罗样激酶-1(Polo-Like Kinase 1,PLK1)蛋白表达与中心体扩增之间的相关性。方法:采用免疫组化SABC法检测10例正常口腔黏膜和47例OSCC中PLK1蛋白和γ-微管蛋白的表达情况,并分析两者之间的相关性。结果:PLK1蛋白和γ-微管蛋白在正常口腔黏膜组和OSCC组中的阳性表达率分别为0.0%(0/10)、70.2%(33/47)和0.0%(0/10)、76.6%(36/47),PLK1蛋白和γ-微管蛋白表达在正常口腔黏膜组和OSCC组之间的差异均具有统计学意义,P<0.05;Spearman相关分析显示,OSCC组织中PLK1蛋白与γ-微管蛋白表达之间正相关,r=0.305,P<0.05。结论:PLK1蛋白过表达可能是中心体扩增的潜在机制之一,PLK1蛋白过表达导致中心体扩增及细胞恶性增殖在OSCC发生中可能起一定的作用。

Objective： To study the correlation between polo-like kinase 1 protein expression and centrosome amplification in oral squamous cell carcinoma. Methods： The expressions of PLK1 and γ-tubulin proteins in 10 cases of normal oral mucosa and 47 cases of oral squamous cell carcinoma were investigated by SABC method, and analyzed the correlation between them. Results： Rate of positive expression of Plkl protein and γ-tubulin in normal oral mucosa group and OSCC group were 0% （0/10）, 70.2% （33/47） and 0% （0/10）, 76.6% （36/47） respectively, and differences of Plkl protein and γ-tubulin expression between OSCC group and normal oral mucosa group were statistically significant, P〈0.05. Spearman correlation analysis showed that the expression of PLK1 protein in OSCC tissue was positively correlated with the expression of gamma tubulin, r=0.305, P〈0.05. Conclusion： Over-expression of PLK1 protein may be a potential mechanism of centrosome amplification, while PLK1 protein leads to centrosome amplification and cell proliferation may play a role in the occurrence of OSCC expression.