丹参血脂茶质量标准研究

Salvia Tea Quality Standard of Lipids

[目的]建立丹参血脂茶中决明子、枳壳定性鉴别及测定丹参酮ⅡA含量方法。[方法]采用TLC对丹参血脂茶中的主要药物决明子、枳壳进行鉴别和高效液相色谱法测定丹参酮ⅡA的含量。[结果]TLC色谱中均能检出决明子、枳壳,专属性强,重现性好;在流动相:甲醇-水(78:22),检测波长270nm,柱温30℃,体积流量1.0m L/min的条件下使用高效液相色谱法对供试品进测定丹参酮ⅡA含量,结果准确可靠,分离完全,重复性好。[结论]TLC与高效液相色谱法简便、专属性强、重现性好,结果稳定,可有效控制丹参血脂茶的质量。

[Objective] To optimize lipid Salvia tea quality standards, establish Salvia lipid tea seed, Citrus aurantium qualitative identification and determination of the content of tanshinone Ⅱ A methods. [Methods] TLC （TLC） Identification of the medicine in Cassia, Citrus aurantium qualitative, simultaneous determination of Tan Ⅱ A content by HPLC. [Results] TLC chromatography could be detected cassia, Citrus aurantium, specific, and reproducible; mobile phase： methanol-water （78：22）, detection wavelength 270 nm, the column temperature was 30℃, the volume flow rate 1.0mL use under/min, high-performance liquid chromatography for the determination of the sample into Tan ii A content, accurate, complete separation and good repeatability. [Conclusion] The method is simple, specific, reproducible, the results are stable, can effectively control the quality of lipids Salvia tea.