高原环境下末梢血和静脉血血常规检测结果差异研究

Research on the difference of routine test results at peripheral blood and venous blood in plateau environment

目的探究分析高原环境下末梢血和静脉血血常规结果之间的差异。方法同时采集210例健康藏族居民的末梢和静脉血标本,对末梢血和静脉血标本进行0 h、2 h、4 h、8 h、24 h、48 h、72 h的血常规检测,并对检验结果进行统计学比较分析。结果 210例末梢血和静脉血即刻检测的血常规结果比较,差异有统计学意义(P<0.05)。末梢血与静脉血WBC、RBC、HGB、MCV、MCH、MCHC、PLT结果有高度的相关性,相关系数分别为0.982、0.987、0.994、0.988、0.992、0.982、0.919、0.832。高原环境室温保存的静脉血48 h时MCV、MCHC,72h时HCT、MCV、MCH、MCHC、PLT结果与即刻检验结果比较,差异有统计学意义(P<0.05),各时间段WBC、RBC、HGB结果与即刻检验结果比较,差异无统计学意义(P>0.05)。结论高原环境低压高寒缺氧,人群的末梢循环差,静脉血标本血常规检验结果相对稳定,建议采集静脉血为宜,偏远牧区室温静脉血标本48 h血常规检测可得到良好稳定的结果。

Objective To explore the difference of routine test results at peripheral blood and venous blood in plateau environ- ment. Methods Peripheral and venous blood samples were collected from 210 healthy Tibetan residents at the same time. The collected peripheral blood and venous blood samples were conducted 0 h, 2 h, 4 h, 8 h, 24 h, 48 h, 72 h of routine blood test, and the test results were analyzed by statistical analysis and comparison. Results The results of routine test of peripheral blood and venous blood in the 210 cases of healthy Tibetan residents were compared and the differences were significant （P 〈 0.05 ）. Test results of peripheral blood and venous blood had a high correlation, and the correlation coefficient of the peripheral blood and venous blood in WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT results were 0.982, 0.987, 0. 996, 0. 988, 0.992, 0. 982, 0. 919 and 0. 832. The results of MCV, MCHC had a significant difference between preservation of venous blood at room temperature 48h in plateau environment and immediate test（P 〈 0.05 ）. The results of HCT, MCV, MCH, MCHC, PLT did not have a significant difference between preservation of venous blood at room temperature of 72 h in plateau environment and imme- diate test （P 〈 0.05 ）. WBC, RBC, HGB results and immediate test results in different periods were compared, and there was no statistical significance on the difference （P 〉 0.05 ）. Conclusion In plateau environment, because of the high altitude low Alpine hypoxia, people have a poor peripheral circulation. The venous blood sample test results are relatively stable, collecting venous blood is appropriate; the remote pastoral areas can keep the venous blood 48 hours until detection, and it can also have a good and stable results.