摘要
为探究Y-氨基丁酸(GABA)对热应激雏鸡小肠黏膜中IL-7和slgA含量的影响,选择体重相近的1日龄文昌雏公鸡,随机分为对照(CK)组、热应激(HS)组、50mg/kgGABA(G50+HS)组和100mg/kgGABA(G100+HS)组,每组36只,每天13:00~15:00将HS组、(G50+HS)组和(G100+HS)组置于(40+0.5)℃人工气候箱中进行热处理,利用ELISA法测定不同周龄雏鸡小肠黏膜液中IL-7和sIgA含量。结果:HS组的十二指肠、空肠及回肠中1L-7和sIn含量显著低于CK组(P〈0.05);(G50+HS)组和(G100+HS)组十二指肠、空肠及回肠中IL-7和sIgA含量显著高于HS组(P〈0.05);(G100+HS)组部分肠段中的IL-7和slgA含量与(G50+HS)组比较有显著差异(P〈0.05)。研究结果表明GABA对热应激造成的肠道损伤具有一定修复作用,能够有效提高热应激状态下雏鸡小肠黏膜的免疫功能,但其保护作用并不与浓度呈正比。
This study was aimed to investigate the effect of dietary GABA on content changes of slgA and IL-7 of intestinal mucosa of chicks under heat stress. One-day-age male Wenchang chicks were randomly divided into CK group,HS group and 50 mg/kg GABA (G50+HS)group and 100 mg/kg GABA (G100+HS)group. Chicks from HS, (G50+HS) and (G100+HS)groups were subjected to heat stress treatment at (40+0.5)~C for 2h every day (13:00 to 15:00). ELISA was used to measure IL-7 and sIgA contents of small intestinal fluid in different-age chicken. The results showed that IL-7 and sIgA of duodenum, jejunum and ileum in HS group were significantly lower than that in CK group (P〈0.05). The IL-7 and sIgA of duodenum,jejunum and ileum in (G50+HS) and (G100+HS) groups were significantly higher than that in HS group (P〈0.05). There was a significant difference in IL-7 and slgA of part of intestinal between (G100+HS) group and (G50+HS) group (P〈0.05). It was concluded that GABA alleviated thenegative effects on development of chick intestinal mucosa, improved the innnune function of intestinal mucosa and played a protective role,but its protective effect was not directly proportional to the concentration.
出处
《中国家禽》
北大核心
2016年第13期22-25,共4页
China Poultry
基金
国家自然科学基金项目(31260555、31560680)