纳米银消毒凝胶抑制配药后输液瓶塞表面细菌生长的研究

Sanitizing gel of silver nanoparticles in inhabitation of bacteria growth on bottle stopper surface after infusion

目的探讨纳米银（silver nanoparticles）消毒凝胶抑制配药后的输液瓶塞表面细菌生长的情况，观察分析配药后90min内接上液体时不再重新消毒瓶口的可行性。方法在输液瓶外盖开启液体内配置药物后，对瓶盖表面保护实施5种方法。观察不同时间不同保护方法对瓶盖表面抑制细菌生长的保护作用效果。结果10个时间段瓶口而经过发生的微生物污染问题，随时间延长，细菌污染（Bacterial contamination，BC）瓶数逐渐升高，菌落总数也逐渐增加，主要污染细菌有以下4种：G＋杆菌、微球菌、类酵母样菌、凝固酶阴性葡萄球菌；纳米银消毒凝胶抑制配药后输液瓶塞表面细菌生长的保护方法能够显著抑制细菌生长，其效果明显优于其他方法，差异具有统计学意义（P〈0．05）。结论纳米银消毒凝胶抑制配药后输液瓶塞表面细菌生长的保护方法可以明显降低输液细菌污染率，这样可以为病人的临床安全输液提供科学的重要保证，有效降低输液反应，减少了医院感染的发生。

Objective To study sanitizing gel of silver nanoparticles in the inhibition of bacteria growth on bottle stopper surface after infusion and to observe and analyze the feasibility that not re-sterilizing bottleneck within 90 min after dispensing medicines. Methods After opening the infusion bottle cover and dispensing medicines, 5 protection methods for bottle cover were carried out. The protective effects of different methods at different times in the inhabitation of bacteria growth on the bottle cover surface were observed. Results Microbial contamination problems were found at 10 different times; more bottles got bacterial contamination and bacterial colonies were getting more as the time ticked on. The main contamination bacteria were G ＋ bacilli, micrococcus, yeast-like fungus, and coagulase-negative staphylococci. Sanitizing gel of silver nanoparticles in the inhibition of bacteria growth on bottle cover surface after infusion was effective and better than the other methods, with statistical differences （P〈0.05）. Conclusions Sanitizing gel of silver nanoparticles in the inhibition of bacteria growth on bottle cover surface after infusion can obviously decrease the infectious rate of infusion fluid, provide the patients with safe infusion, effectively reduce infusion reactions, and reduce hospital infections.