实时荧光定量RT-PCR法比较猪瘟疫苗病毒含量

Comparison of virus content in swine fever vaccine by fluorogenetic quantitative RT-PCR

为有效检测福建省使用猪瘟兔化弱毒疫苗病毒含量,给养殖户选择高效疫苗提供科学指导。本研究收集福建省内使用的10个不同厂家生产的23个批次猪瘟疫苗,应用实时荧光定量RT-PCR方法检测疫苗中病毒核酸的含量,以其中的标准品对照,比较相应的兔体反应量。结果表明:抽检23个猪瘟疫苗样品合格率仅为69.56%,传代细胞苗的病毒含量相对较高,脾淋苗病毒含量优于细胞苗;不同厂家生产的同类型疫苗病毒含量差异大,同一厂家生产的同类疫苗存在批间差异。实时荧光定量RT-PCR检测猪瘟疫苗病毒含量是一种便捷、高效的方法,为今后临床猪瘟疫苗的使用提供有效指导依据。

To effectively detect the quantity of the viral in swine vaccine in Fujian province, select high effective vaccine for the farms,23 batches vaccine from 10 different companies collected and used real-time PCR to detect the quantity of the viral. Using standard substance as control, we compared the corresponding RID. The result showed that the percent of pass in 23 batches vaccine is only69.5%. The quantity of viral in passaged cell vaccine is relative high and the quantity of the viral in spleen vaccine is better than others. There is a big differences between different companies and between different batches in one company. Real-time PCR is an convenient and effective method to detect the quantity of the viral in swine vaccine and can provide useful guideline for selecting vaccine.