鹅脂肪甘油三酯脂肪酶和长链脂酰辅酶A合成酶1基因表达差异及其对脂肪沉积和血清脂类代谢的调控

Differential Expression of Adipose Triglyceride Lipase and Long-Chain Acyl-CoA-Synthetase 1 Genes and Their Regulation of Fat Deposition and Serum Lipid Metabolism of Geese

本试验旨在研究脂肪甘油三酯脂肪酶(ATGL)和长链脂酰辅酶A合成酶1(ACSL1)基因在鹅的不同组织器官中的表达差异,并探索2个基因表达对机体脂肪沉积和血清脂类代谢的调控。选取16周龄五龙鹅30只(公母各占1/2),屠宰后用实时荧光定量PCR检测不同组织器官(肝脏、心脏、皮下脂肪、腹部脂肪、胸肌、腿肌、肌胃、腺胃、小肠、肾脏、大脑、肺、脾脏)中A TG L、A CSL1基因表达量。结果表明:1)在鹅的皮下脂肪、腹部脂肪、肝脏、脾脏、肾脏、心脏、胸肌和腿肌中均检测出ATGL和ACSL1基因的表达;ATGL基因在皮下脂肪和腹部脂肪中表达量最高,其次是肝脏和脾脏,在肾脏、心脏、胸肌和腿肌中只有少量表达;ACSL1基因在皮下脂肪、腹部脂肪、肝脏、脾脏中表达量较高,在肾脏、心脏、胸肌和腿肌中有少量表达,而在肌胃、腺胃和肺中几乎不表达。2)ATGL基因表达量与腿肌肌内脂肪率、胸肌肌内脂肪率、腹部脂肪率、胸肌率和腿肌率呈显著或极显著负相关(P<0.05或P<0.01),与皮下脂肪率呈显著正相关(P<0.05);ACSL1基因表达量与腿肌肌内脂肪率、胸肌肌内脂肪率、胸肌率呈正相关(P>0.05),与腿肌率呈显著正相关(P<0.05),与皮下脂肪率呈显著负相关(P<0.05)。3)ATGL基因表达量与血清甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和葡萄糖含量呈显著或极显著正相关(P<0.05或P<0.01);ACSL1基因表达量与血清总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和葡萄糖含量呈负相关(P>0.05),与甘油三酯含量呈显著负相关(P<0.05)。由此可见,ATGL和ACSL1基因在鹅的不同组织器官中的表达具有明显差异性,对机体脂肪沉积和血清脂类代谢具有反向调控作用。

This experiment was conducted to study the difference of adipose triglyceride lipase（ ATG L） gene and long-chain acyl-Co A-synthetase 1（ ACSL1） genes expression in different tissues and organs of geese,and to explore the expression of tw o genes on body fat deposition and serum lipid metabolism regulation. Selected thirty 16-w eek-old Wulong Geese（ half male and half female）,and the ATG L and ACSL1 gene expressions in different tissues and organs（ liver,heart,subcutaneous fat,abdominal fat,breast muscle,leg muscle,muscular stomach,glandular stomach,small intestine,kidney,brain,lung,spleen） were detected by real-time quantitative PCR after slaughter. The results show ed as follow s： 1） The expressions of ATG L and ACSL1 genes were detected in subcutaneous fat,abdominal fat,liver,spleen,kidney,heart,breast muscle and leg muscle of geese; ATG L gene expression in subcutaneous fat and abdominal fat relative was the highest,follow ed by that in the liver and spleen,in kidney,heart,breast muscle and leg muscle only a few of expression. ACSL1 gene expression in subcutaneous fat,abdominal fat,liver and spleen was the highest,and slightly expressed in kidney,heart,breast muscle and leg muscle,and almost no expression in muscular stomach,glandular stomach and lung. 2） ATG L gene expression show ed a significant negative correlation with intramuscular fat（ IMF）percentage in leg muscle,IMF percentage in breast muscle,percentage of abdominal fat,percentage of leg muscle and percentage of breast muscle（ P〈0. 05 or P〈0. 01）,and was significant positively correlated with percentage of subcutaneous fat（ P〈0.05）; ACSL1 gene expression was positively related with IMF percentage in leg muscle,IMF percentage in breast muscle and percentage of breast muscle（ P〈0. 05）,and was significantly positively correlated with percentage of leg muscle（ P〈0. 05）,while it show ed a significant negative correlation with percentage of subcutaneous fat（ P〈0. 05）. 3） The ATG L gene expression was significantly positively correlated with the contents of total cholesterol（ TCH）,high density lipoprotein cholesterol（ HDL-C）,low density lipoprotein cholesterol（ LDL-C） and glucose（ GLU） in serum（ P〈0. 05 or P〈0. 01）; the A CSL1 gene expression was negatively correlated with the content of TCH,HDL-C,LDL-C and GLU in serum（ P〈0.05）,and it were significantly negatively correlated with the triglyceride content. In conclusion,the expressions of ATG L and ACSL1 genes in different tissues and organs of geese have obvious differences,which have a negative regulation on fat deposition and serum lipid metabolism.