摘要
采用免疫荧光标记法结合共聚焦显微镜(CLSM)研究了自水解强度对杨木聚木糖分布的影响。结果表明,聚木糖荧光信号较均匀地分布于杨木纤维细胞壁中,随强度因子的增大细胞壁中心部位荧光信号下降逐渐增多,细胞壁边缘荧光信号下降较少,有些部位甚至会稍许增加。采用高效液相色谱(HPLC)检测法测得强度因子增强到3.72时,聚木糖含量从原料的14.64%下降到7.33%;对聚木糖荧光图谱进行区域强度统计,可得出与HPLC检测法结果相同的变化趋势。
The change of xylan distribution in the cell wall of poplar chips caused by auto-hydrolysis pretreatmeut was studied by using immunofluorescence combined with eonfocal laser scanning microscopy (CLSM). A gradual loss of xylan antibody signals in the center of the poplar cell wall was observed but only a slight loss at the edge or even a slight increase at some places of the cell wails were found as the intensity factor of auto-hydrolysis pretreatment increased. HPLC analysis was also conducted to measure the overall change of xylan content. As the intensity factor increased to 3.72, xylan content decreased from 14. 64% to 7.33%. The change of the xylan signal intensity from spectra of the CLSM had the same trend as that of the xylan content determined by using the HPLC analysis.
出处
《中国造纸》
CAS
北大核心
2016年第7期1-6,共6页
China Pulp & Paper
基金
国家自然科学基金项目(31270630)的资助
关键词
自水解预处理
杨木聚木糖
免疫荧光标记法
共聚焦显微镜
auto-hydrolysis pretreatment
xylan in poplar chips
immunofluorescence
confocal laser scanning microscopy