小分子化合物诱导茶树抗虫性相关生理指标的变化

Changes of Insect-resistance Relative Physiology Indexes Induced by Low Molecular Compounds in Tea Plant

以麝香草酚等10种小分子物质作为激发剂,比较研究了它们诱导茶树与害虫防御有关的酶系统变化和茶树主要生化成分变化的特征,以了解它们所引起的诱导反应的差异。结果表明,各诱导物质几乎一致性地引起茶树叶片可溶性蛋白的上升,除3-氨基丁酸处理外各处理叶片氨基酸总量也趋于上升。水杨酸、磺基水杨酸、对羟基苯甲酸乙酯、2,5-二羟基苯甲酸在结构上具有高度共性,但它们所引起的生理反应却有明显差异。与水杨酸相比,磺基水杨酸、对羟基苯甲酸乙酯、2,5-二羟基苯甲酸诱导的茶树叶片生化物质的变化幅度较大,2,5-二羟基苯甲酸诱导处理下叶片过氧化物酶(POD)和多酚氧化酶(PPO)活性明显上升,有一定应用潜力。Harpin蛋白处理引起苯丙氨酸解氨酶(PAL)活性显著下降,BTH处理引起PAL活性显著上升,但二者都未引起叶片多酚含量的显著变化,提示PAL与多酚的关系还有更多需要了解。百里香酚和肉桂醛处理引起POD和PPO活性下降,但诱导叶片咖啡碱含量的明显上升,可能对茶树具有特殊生理作用。

Taken 10 low molecular compounds（ thymol, cinnamyl aldehyde, salicylic acid （SA）, sulfusalicylic acid, ethyl p-hydroxybenzoate, 3-amino-butyricacid（BABA） , benzothiadiazole（BTH）, 2,5-dihydmxybenzoic acid, methyl jasmonate, harpin） as activators, charac- teristics of the induced changes of protect enzymes as well as the main bio-chemical components in tea plant were checked. The results showed that all activators could induce the increase of soluble protein content in tea plant except for 3-amino-butyrieacid, and all other activatom could also induce the increase of amino acid in tea leaves. On the other side though salicylic acid, sulfosalicylic acid, ethyl p-hydroxy- benzoate, 2,5-dihydroxybenzoic acid were similar in molecular structure but induced a much different change of the main hie-chemicals in tea plant. Compared with salicylic acid, the variation amplitude of bio-chemieals induced by sulfosalicylic acid, ethyl p-hydroxybenzeate, 2, 5-dihydroxybenzoic acid was greater,and 2,5-dihydroxybenzoie acid also induced the increase of the enzyme activity of POD and PPO obvi- ously. Harpin inducing activity of PAL decreased sharply, while BTH inducing the PAL activity increased distinctly, but the polyphenol contents changed a little under the treatments of both activators, which indicated that there still were a lot of things to be understand between the relationship of PAL and tea polyphenols. With thymol, cinnamyl aldehyde inducing, the POD, PPO activities of tea plant decreased but the coffine content in tea leaves increased, which indicated that these two materials might have some special physiology function on tea plant.